健脾清化方对不同饮食喂养的CRF大鼠胃动素、胃泌素的影响

目的观察健脾清化方对不同饮食喂养的慢性肾衰竭(CRF)大鼠胃动素、胃泌素的影响,探讨其改善慢性肾功能衰竭的胃肠道症状,降低血肌酐,延缓肾功能衰竭进展的机制。方法将80只SD雄性大鼠随机分为正常组8只,造模组72只,应用Platte法切除大鼠肾组织制作慢性肾功能衰竭动物模型,按不同饮食喂养分为7组,喂养8周后处死并采集标本,测定血肌酐、尿素氮、24h尿蛋白定量、胃动素、胃泌素等指标,进行分析。结果各造模组血肌酐、尿素氮和24h尿蛋白定量均显著高于正常组(P<0.05),其中高脂饲料模型组和持续高脂饲料模型组的胃动素、胃泌素明显高于正常组(P<0.05,P<0.01)。健脾清化方不仅具有显著降低持续高脂饮食饲料模型组的血肌酐、尿素氮及24h尿蛋白定量的作用(P<0.01),同时能明显抑制胃动素、胃泌素的分泌(P<0.05)。结论健脾清化方具有明显抑制胃动素、胃泌素,改善慢性肾功能衰竭的胃肠道症状和延缓肾功能衰竭进展的作用。

Effects of "Jianpi Qinghua Decoction" on Motilin and Gastrin in Chronic Renal Failure Rats by Different Fat Feeds

Objective To observe the effects of "Jianpi Qinghua Decoction" on motilin and gastrin and its mechanisms in improving gastrointestinal symptoms, reducing Scr and delaying CRF progress in chronic renal failure(CRF) rats by different fat feeds. Methods Establish three CRF rat models by undergoing 5/6 nephrectomy and supplying different fat feeds. After eight weeks of treatment with distilled water, "Jianpi Qinghua Decoction" and Monopril, the levels of 24-h urine protein(Upr), blood urea nitrogen(BUN) and motilin and gastrin of rats in these three models were examined. Results The levels of 24-h Upr,BUN and serum Cr in the three models were significantly higher than those in the untreated groups. The levels of motilin and gastrin of two models by supplying high-fat feeds were significantly higher than the model by supplying normal-fat feed. The levels of 24-h Upr in Decoction-treated groups of three models were significantly lower than those in the untreated group of three models. The levels of motilin and gastrin in Decoction-treated groups of two models by supplying high-fat feed were significantly lower than those in the untreated groups of two models by supplying high-fat feed. Conclusion This Decoction can suppress the secretion of motilin and gastrin to improve gastrointestinal symptoms and delay CRF progress.