| 小鼠胸腺上皮细胞株MTEC_1细胞自发分泌CSF样生物活性因子 |
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| 引用本文： | 张平夏,曹良弦,陈慰峰,吴江声.小鼠胸腺上皮细胞株MTEC_1细胞自发分泌CSF样生物活性因子[J].北京大学学报(医学版),1992(1). |
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| 作者姓名： | 张平夏 曹良弦 陈慰峰 吴江声 |
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| 作者单位： | 北京医科大学免疫学教研室
(张平夏，曹良弦，陈慰峰)，北京医科大学组织学胚胎学教研室(吴江声) |
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| 摘 要： | 用~3H-TdR参入法及半固体琼脂培养法,检测出本室自建小鼠胸腺基质细胞林中的M TEC_1能自发分泌集落刺激因子(CSF)样生物活性因子。用抗IL-6单抗不能封闭MTEC_1-SN的支持骨髓细胞增殖的作用。根据细胞形态学分析,MTEC_1-SN主要促进CFU-GMM和CFU-GM形成。表明MTEC_1细胞除能自发分泌IL-1及IL-6外,尚能自发分泌CSF样活性因子,其主要成份可能为GM-CSF。
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| 关 键 词： | 胸腺上皮细胞株 骨髓干细胞 集落刺激因子 |
SPONTANEOUS SECRETION OF COLONY STIMULATING-LIKE FACTORS BY MURINE THYMUS EPITHELIAL CELL LINE MTEC_1 CELLS |
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| Zhang Pingxin,et al.SPONTANEOUS SECRETION OF COLONY STIMULATING-LIKE FACTORS BY MURINE THYMUS EPITHELIAL CELL LINE MTEC_1 CELLS[J].Journal of Peking University:Health Sciences,1992(1). |
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| Authors: | Zhang Pingxin |
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| Institution: | Zhang Pingxin,et al Department of Immunology |
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| Abstract: | MTEC_1 cell line, a type of murine thymus epithelial cells established in our laboratory, spontaneo-usly secreted colony stimulating-like factors. FATEC_1-SN sustained the proliferation and colony unitformation of murine bone marrow cells in a dosedependent fashion. The biological activity of colony sti-mulating-like factors produced by MTEC_1 could only be neutralized slightly by anti-IL-6 McAb. Thedominant types of colonies induced by MTEC_1-SN were mixed granulocyte/monocyte/megakaryocy co-lonies (CFU-GMM) and granulocyte/macrophage colonies(CFU-GM). It suggests that MTEC1_-SN conta-ins CSF-like factor(s). |
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| Keywords: | Mouse thymus epithelial cell line Bone marrow stem cell Colony-stimulating factor |
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