原因不明复发性流产患者巨噬细胞表面TSP1、CD36、CD47的表达及其细胞因子的分泌

目的:探讨巨噬细胞表面分子凝血酶敏感蛋白1(TSP1)、CD36、CD47及其分泌细胞因子IL-10和IFN-γ与原因不明复发性流产发病的关系。方法:采用流式细胞技术检测10例原因不明复发性流产患者和20例正常早孕妇女外周血及蜕膜巨噬细胞表面TSP1、CD36、CD47的表达;同时用ELISPOT法检测两组外周血及蜕膜分泌IL-10和IFN-γ的巨噬细胞数。结果:原因不明复发性流产患者外周血巨噬细胞TSP1、CD36、CD47表达水平及其分泌IL-10和IFN-γ的细胞数与正常早孕妇女均无统计学差异。与正常早孕妇女比较,原因不明复发性流产患者蜕膜巨噬细胞CD36表达水平明显升高(P<0.01),TSP1表达水平明显降低(P<0.01),CD47表达水平无明显差异(P>0.05)。蜕膜组织分泌IL-10的巨噬细胞数显著降低(P<0.05),而分泌IFN-γ的巨噬细胞数量无明显差异。结论:蜕膜巨噬细胞在维持正常妊娠免疫耐受和导致原因不明复发性流产发病中起重要作用。

Expression of TSP1,CD36,CD47 and cytokine secretion on macrophage of women with unexplained recurrent spontaneous abortion

Objective:To investigate the relationship between expression of TSP1,CD36 ,CD47 and secretion of IL-10,IFN-γ on macrophage and the occurrence of unexplained recurrent spontaneous abortion (URSA).Methods:There were 20 women with selective termination of normal early pregnancy and 10 women with URSA that involved in the research.Flow cytometry was used to detect the expression of TSP1,CD36,CD47 on macrophages in peripheral blood and decidua.Cytokine secretion of macrophages was detected by enzyme-linked immunosorbent spot-forming (ELISPOT) cell assay.Results:The expression of TSP1,CD36,CD47 and secretion of IL-10,IFN-γ on macrophage in peripheral blood had no difference between the two groups.The expression of CD36 was increased significantly (P<0.01) and the expression of TSP1 was decreased significantly (P<0.01) on decidual macrophages of women with URSA when compared with that of women with normal pregnancies.There was no difference in the expression of CD47 on decidual macrophage between the two groups.The percentage of IL-10 secreting cells in decidual macrophages of women with URSA was significantly higher than that of women with normal pregnancies (P<0.05).There was no difference in the percentage of IFN-γ secreting cells in decidual macrophages between these two groups.Conclusion:Macrophage is one of the most important immunologic cells at maternal-fetal interface,which may play an important role in maintenance of normal pregnancy and pathogenesis of URSA.