前列腺素E_2及硝苯吡啶对某些免疫功能的影响

PGE_23×10~(-7)～3×10~(-6)mol/L及硝苯吡啶5×10~(-6)～1×10~(-5)mol/L对ConA诱导的淋巴细胞增殖有明显抑制作用。药物作用48h后洗去药物,加入致裂原后继续培养细胞,结果发现硝苯吡啶的抑制作用已完全逆转,而PGE_23×10~(-6)mol/L的抑制作用则部分逆转。当低浓度PGE_2(3×10~(-3)mol/L)与硝苯吡啶(1×10~(-6)mol/L)同时作用于脾细胞时,可见两者作用正巧相加,无明显拮抗与协同作用。PGE_26×10~(-6)mol/L及硝苯吡啶1×10~(-5)mol/L对巨噬细胞移动有直接抑制作用。用~(51)Cr标记调理的绵羊红细胞方法证明低浓度PGE_2对巨噬细胞吞噬功能有增强,而硝苯吡啶预培养对吞噬功能影响不大。

EFFECTS OF PGE_2 & NIFEDIPINE ON SOME IMMUNOLOGIC FUNCTIONS

In this study,we observed that PGE2 3×10-7~3×10-6 mol/L & nifedipine 1 ×10-5mol/L inhibited lymphocyte proliferation markedly.The effect of nifedipine was completely reversible, however the effects of PGE2(3 ×10-6mol/L)was only partly reversible .In combination of PGE2(3×10-8mol/L) with nifedipine (1 ×10-6mol/L) ,the effect was additive,no significant antagonism and synergism were observed .PGE26× 10-6mol/L and nifedipine 1×10-3 mol/L directly inhibited maerbphage migration but no significant effect on PHA-induced MIF .Preincuba-tation with nefidipine did not influonce macrophage phagocytosis, but PGE2 slightly enhanced it. The results stated above strongly suggests that PGE2 may regulate cells interaction and Ca2+ is required during lymphocyte proliferation.