乳腺癌腋淋巴结微小转移与癌组织c-erbB-2、nm23基因表达关系的研究

目的 探讨乳腺癌腋淋巴结微小转移与c-erbB-2、nm23基因表达的关系。方法 采用免疫组织化学S-P法对70例淋巴结常规HE染色无癌转移的乳腺癌腋淋巴结1141枚进行上皮细胞角蛋白19（CK-19）表达和原发癌组织进行c-erbB-2、nm23基因表达的检测。结果 淋巴结CK-19阳性率为4.21%（48/1141）,其病例阳性率为27.14%（19/70）。c-erbB-2阳性率为44.29%（31/70）,nm23阳性率为80.00%（56/70）.CK-19阳性组的c-erbB-2阳性率明显高于CK-19阴性组﹙P＜0.05﹚,CK-19阳性组的nm23阳性率明显低于CK-19阴性组﹙P＜0.05﹚。CK-19阳性率与病人月经状况、病理类型、临床分期、激素受体状况无关（P＞0.05）。结论 乳腺癌腋淋巴结CK-19表达与癌组织c-erbB-2基因表达呈正相关,与癌组织nm23基因表达呈负相关,与病人月经状况、临床分期、病理类型、激素受体状况无关。

The relationship of micrometastasis in axillary lymph node and c-erbB-2, nm23 gene in breast cancer

Objective To investigate micrometastasis in axillary lymph nodes and its relationship with c-erbB-2, nm23 genes expression of the tumor tissue in breast cancer. Methods Total of 1141 lymph nodes and carcinoma tissues from 70 lymph node-negative cases comfirmed by routine pathology were examined by immunohistochemical staining using CK-19 and c-erbB-2, nm23 respectively. Results The positive rate of CK-19 of axillary lymph node was 4.21%(48/1141). Micrometastasis were observed in 19 cases of the 70 patients(27.14%). The positive rate of c-erbB-2 was 44.29%(31/70), the positive rate of nm23 was 80.00%(56/70) in breast cancer tissues. The positive rate of c-erbB-2 in the patients with micrometastasis was significantly highr than that in the patients without micrometastasis(P<0.05). The positive rate of nm23 in the patients with micrometastasis was significantly lower than that in the patients without micrometastasis(P<0.05). There was no significant relationship with patient menstruation condition, clinical stage, pathologic type, hormone condition irrelevant to CK-19 expression. Conclusin The expression of CK-19 of axilliariy lymph node is positive relevant to c-erbB-2 gene with cancer, neganative relevant to nm23 gene with cancer. The expression of CK-19 of axilliariy lymph node is irrelevant to patient menstruation condition, clinical stage, pathologic type, hormone condition.