大蒜素对金属基质蛋白酶表达的影响

目的：研究大蒜素对高脂血症家兔金属基质蛋白酶（MMP-13）表达的影响。方法：选用健康新西兰雄性大白兔36只，体重2．24-0．3。随机将36只新西兰雄性大白兔分为三组：（1）空白对照组（正常组）12只，每天喂食普通饲料；（2）阳性对照组（模型组）12只，每天喂食1g胆固醇＋10％猪油＋普通饲料；（3）大蒜素组（治疗组）12只，每天喂食1g胆固醇＋10％猪油＋普通饲料＋8mg／kg大蒜素。实验开始时，每天7：00治疗组腹腔注射大蒜素，其他两组腹腔注射等量的生理盐水2．5mg／kg。在第16周末，测量血脂指标（TC、TG、HDL—C、LDL—c）以及脂质过氧化产物丙二醛（MDA）、超氧化物歧化酶（SOD）的指标后，处死全部家兔。取主动脉标本观察并记录其形态学结果后进行HE染色观察并记录标本的病理变化，通过免疫组织化学方法观察并记录标本斑块内金属基质蛋白酶（MMP-13）的表达情况。结果：大蒜素能够降低血中TC、TG、LDL—C、升高HDL—C以及减少丙二醛的含量，并提高超氧化物歧化酶SOD的活力，同时能够降低斑块中金属基质蛋白酶（MMP-13）的阳性表达率。减小已经形成的动脉粥样硬化斑块的作用。结论：大蒜素具有降低血脂，抗脂质过氧化，降低斑块中金属基质蛋白酶（MMP-13）的阳性表达率，预防动脉粥样硬化形成的作用外，还对动脉粥样硬化斑块的消退有一定的影响。

THE EFFECT OF GARLICIN ON THE EXPRESSION OF MMP-13 LEVEL

Objective：To investigate the effect of garlicin on the expression of MMP - 13 level in sample atherosclerosis （AS） plaques. Methods：Thirty six healthy male New Zealand white rabbits （weight ：2.2 ± 01. 3kg） were randomly divided into 3 groups, 12 rabbits in every group ： （ 1 ） 览 control group （ normal group）, fed with general feed every day, （ 2 ） positive control group （ model group）, fed with 1 g cholesterol, 10% lard and general feed, （3）Garlicin group（ prophylaxis group）, fed with 1 g cholesterol, 10% lard,8 mg/ kg garlicin and general feed. From the beginning of the experiment, positive control group was given 2.5 mg/kg garlicin celiac injection,and other groups were given 2.5 mg/kg saline water celiac injection every day. At the end of 16 weeks,the levels of serum TC , TG , LDL - C , HDL - C , MDA and SOD were measured. Then all rabbits were kill and the MMP - 13 level in sample atherosclerosis plaques were studied by means of immunohistochemistry. Results： It was found that garlicin could decrease the levels of the serum TC , TG , LDL - C and MDA in garlicin group compared with those in positive control group,increase the serum HDL - C and SOD concentration, decrease the rate of positive expression of MMP- 13 level in sample AS plaques and alleviate the atherosclerotic lesion. Conclusion： garlicin can cure the formatted atherosclerosis plaques besides lowering serum lipids, decreasing the peroxidation of lipids and the rate of positive expression of MMP - 13 level in sample AS plaques and preventing the formation of atherosclerosis.