乳腺癌中ERK2、nm23、MMP9基因蛋白的表达及意义

目的探讨乳腺癌中ERK2基因蛋白表达与淋巴结转移相关基因nm23、MMP9蛋白表达的关系。方法采用HE染色光镜观察96例乳腺癌,用免疫组织化学技术检测病灶中ERK2、nm23、MMP9蛋白表达情况。结果ERK2蛋白表达在乳腺浸润性癌中有高于原位癌的趋势（χ^2=3.783,P=0.052）;nm23蛋白表达在淋巴结转移组中显著低于无淋巴结转移组（χ^2=9.222,P=0.010）,与ERK2蛋白表达呈显著负相关（χ^2=4.085,P=0.043）;MMP9蛋白表达在淋巴结转移组中显著性高于无淋巴结转移组（χ^2=4.153,P=0.043）,与ERK2蛋白表达无明显相关性（P〉0.05）。结论ERK2信号蛋白可能通过与nm23基因蛋白的作用影响乳腺癌的进展、淋巴结转移。

Expression and Signifcance of ERK2,nm23 and MMP9 in Breast Cancer

Objective To investigate the correlation of the expression of ERK2 with MMP9 and nm23 in patients with breast cancer. Methods The expression of ERK2 , nm23 and MMP9 was detected by immunohistochemical method. Results There was a tendency of higher expression rate of ERK2 in invasive breast cancer than in the carcinoma in situ （χ^2= 3. 783 ,P = 0.052）. The positive rate of nm23 protein expression in node metastasis group was significantly lower than that in no metastasis group（ χ^2= 9.222 ,P = 0. 010）. nm23 protein expression was negatively correlated with ERK2 protein expression （χ^2= 4.085, P = 0.043 ）. The positive rate of MMP9 protein expression in patients with node metastasis was significantly higher than that without node metastasis （χ^2= 4. 153, P = 0. 043 ）, but was not significantly correlated with the ERK2 protein expression of breast cancer（P 〉 0.05 ）. Conclusion ERK2 might be involved in the progression and node metastasis of breast cancer by regulating the nm23 protein.