正交实验优选益视口服液纯化工艺

目的：考察壳聚糖澄清剂用于益视口服液的纯化工艺。方法以益视口服液中2，3，5，4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的转移率及干膏率为考察指标，采用 L9（34）正交试验设计，优选壳聚糖絮凝沉降法的纯化工艺条件；并对纯化前后药液的黏度、浊度、蛋白质及鞣质含量进行测定。结果壳聚糖絮凝沉降法的最佳条件是药液质量浓度是1.0 g / mL，壳聚糖的加入量为30％，药液 pH 4，温度是40℃，经纯化后黏度、浊度、蛋白质及鞣质含量均有所下降。结论壳聚糖絮凝沉降法可用于益视口服液的纯化。

optimization of The Chitosan purification process of Yishi oral Solution by orthogonal Test

objective To investigate purification technology of Yishi Oral liquid with chitosan as clarification agent. Method The transfer rate of 2,3,5,4’-styrene-4-hydroxy -2-O-β-D - glycosidase and extraction rate as indexes,the best purification process conditions of chitosan flocculation settlement method were optimized by using L9(34 )orthogo-nal experiment design,and the viscosity,turbidity,the content of protein and tannin were determinated before and af-ter purification liquid. Result Optimum purification process of chitosan flocculation sedimentation method was:the mass concentration of liquid1. 0 g / mL,the addition amount of chitosan 30% ,pH 4 at 40 ℃. The viscosity,turbidity , the content of protein and tannin were decreased after purification. Conclusion Chitosan flocculation settlement method can be used for purification of Yishi Oral Solution.