反相高效液相色谱法测定人血浆中克林霉素浓度

目的建立反相高效液相色谱法测定人血浆中克林霉素浓度的方法。方法采用反相高效液相色谱法，NUCLEODUR C18柱（150mm×4．6mm，5μm），流动相为乙腈-四氢呋喃-磷酸缓冲液（27：1：72），流速：1mL·min^-1，测定波长：204nm。结果克林霉素最低定量浓度为0．05μg·mL^-1，线性范围为0．05-16μg·mL^-1（r=0．9983），方法回收率均〉95％。日内、日间精密度（RSD）〈10%。结论该方法快速、简便、无杂质干扰，适用于克林霉素的体内药物浓度测定及药动学研究。

Determination of clindamycin in human plasm by high performance liquid chromatography

Objective To establish a reversed phase high-performance liquid chromatography method to determine clindamycin in the human plasma. Methods RP-HPLC was used with NUCLEODUR C18 column （150 mm×4.6 mm, 5 μm）, and acetonitrile-furan-H3PO4 buffer （27 ： 1 ： 72） was used as the mobile phase. The detection wavelength was 204 nm and the flow rate was 1 mL · min^-1. Results The calibration curve was linear within 0.05 - 16μg · mL^-1 （r=0. 998 3）. The method recoveries were higher than 95%. The RSD values of intra-day and inter-day were lower than 10%. Conclusion The method is accurate, sensitive, specific and reliable for clindamycin. It may be used to study the pharmacokinetics of clindamycin.