| 曲古菌素A通过抑制MAPK/ERK通路上调食管癌EC1细胞CAR的表达 |
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| 引用本文： | 马俊芬,刘康栋,刘 霞,杨洪艳,黄幼田,赵明耀,董子明.曲古菌素A通过抑制MAPK/ERK通路上调食管癌EC1细胞CAR的表达[J].中国肿瘤生物治疗杂志,2010,17(2):139-143. |
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| 作者姓名： | 马俊芬 刘康栋 刘 霞 杨洪艳 黄幼田 赵明耀 董子明 |
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| 作者单位： | 郑州大学,基础医学院,病理生理学教研室,河南,郑州,450052 |
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| 基金项目： | 教育部科技创新工程基金资助重点项目（No. 207150） |
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| 摘 要： | 摘 要 目的：观察曲古菌素A（trichostatin A，TSA）对人食管癌细胞EC1膜表面柯萨奇病毒-腺病毒受体(Coxsachievirus and adenovirus receptor, CAR)表达水平的影响，探讨MAPK/ERK信号通路在TSA上调CAR表达中的作用。方法：0.3、0.5、1.0 μmol/L的TSA处理EC1细胞48 h，采用免疫荧光、RT-PCR、Western blotting检测CAR的表达。以1.0 μmol/L TSA作用EC1细胞1、6、12、24、48 h，Western blotting检测p-ERK、CAR表达水平的变化，分析CAR表达和p-ERK水平变化的相关性。结果：0.3、0.5、1.0 μmol/L TSA处理EC1细胞后，CAR蛋白和mRNA水平均明显增加（P<0.05)，并呈剂量依赖关系。1.0 μmol/L TSA作用EC1细胞6、12、24、48 h后，CAR蛋白表达较对照组均明显增加（P<0.05)；p-ERK表达水平均明显下降（P<0.05)，两者变化呈显著负相关（r=-0.886，P<0.01)。结论：TSA能够上调人食管癌EC1细胞膜表面CAR的表达水平，其机制可能与其抑制MAPK/ERK通路有关。
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| 关 键 词： | 食管癌细胞 曲古菌素A 柯萨奇病毒-腺病毒受体 细胞外信号调节激酶 |
| 收稿时间： | 2009/12/29 0:00:00 |
| 修稿时间： | 2010/2/25 0:00:00 |
Trichostatin A up-regulates expression of Coxsachievirus and adenovirus receptor in human esophageal cancer EC1 cells through inhibiting MAPK/ERK pathway |
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| MA Jun-fen,LIU Kang-dong,LIU Xi,YANG Hong-yan,HUANG You-tian,ZHAO Ming-yao and DONG Zi-ming.Trichostatin A up-regulates expression of Coxsachievirus and adenovirus receptor in human esophageal cancer EC1 cells through inhibiting MAPK/ERK pathway[J].Chinese Journal of Cancer Biotherapy,2010,17(2):139-143. |
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| Authors: | MA Jun-fen LIU Kang-dong LIU Xi YANG Hong-yan HUANG You-tian ZHAO Ming-yao and DONG Zi-ming |
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| Institution: | Department of Pathophysiology, Basic Medical College of Zhengzhou University, Zhengzhou 450052, Henan, China;Department of Pathophysiology, Basic Medical College of Zhengzhou University, Zhengzhou 450052, Henan, China;Department of Pathophysiology, Basic Medical College of Zhengzhou University, Zhengzhou 450052, Henan, China;Department of Pathophysiology, Basic Medical College of Zhengzhou University, Zhengzhou 450052, Henan, China;Department of Pathophysiology, Basic Medical College of Zhengzhou University, Zhengzhou 450052, Henan, China;Department of Pathophysiology, Basic Medical College of Zhengzhou University, Zhengzhou 450052, Henan, China;Department of Pathophysiology, Basic Medical College of Zhengzhou University, Zhengzhou 450052, Henan, China |
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| Abstract: | |
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| Keywords: | esophageal cancer cell trichostatin A Coxsachievirus and adenovirus receptor(CAR) extracellular signal-regulated kinase (ERK) |
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