磷酸钙骨水泥/骨形态发生蛋白复合人工骨的生物相容性

目的：将自行合成的磷酸钙骨水泥（CPC）作为载与BMP复合成人工骨，检测其生物相容性，方法：制备CPC／BMP及CPC骨块，免疫原性，对血液系统的影响街道一物相容性指标，结果：动物实验表明材料属无毒级，不含致热原，体外试验不引起溶血反应，对凝血功能无明显影响，植入兔或小鼠肌内未检测出特异性抗体，组织学检查未见免疫排斥反应，对肌肉无刺激作用，对体外培养的细胞增殖没有明显抑制作用，结论：材料有较好的生物相容性，临床使用安全。

Biocompatibility of calcium phosphate cement/BMP composite as bone graft material

AIM To construct CPC/BMP composite by combining CPC with BMP, and to detect their biocompatibity. METHODS The toxicity, pyrogen, hemolytic activities and immunogen were detected by means of animal test, cell culture, hemolysis test and histochemical technique. RESULTS The composites were found no toxicity and no pyrogen by animal test, no hemolytic activities and couldn't influence the coagulate of blood in vitro . When the materials were grafted into the muscle pounches in the thigh of mice or rabbits, no obvious specific antibodies produced were detected in serum by ELISA, nor were significant immune reaction of foreign body observed by histochemistry technique. The composites were also testified no cytotoxicity in vitor for the proliferation of cultured cells couldn' be inhibited. CONCLUSION The CPC/BMP composite might have good biocompatibility and be safe for clinical use.