全自动血培养仪中布鲁菌阳性报警时间及与其他病原菌的比较

目的通过探讨布鲁菌实验室培养特性，分析在BACTECTM9120全自动血培养仪中布鲁菌的阳性报警时间,提高对布鲁菌的认识。方法对某院分离的76株布鲁菌的阳性报警时间进行统计分析，并将其与其他病原菌的阳性报警时间进行比较。结果76株布鲁菌，以血液来源为主（66株，86.85%），其次是骨髓（7株，9.22%），脓液、脑脊液、关节腔液各1株（1.31%）。布鲁菌阳性报警时间主要分布在72～120 h，来源于骨髓标本的布鲁菌平均阳性报警时间为(84.51±8.86) h，短于来源于血液标本的(98.27±10.32) h（t=3.39，P＜0.01）。布鲁菌平均阳性报警时间为(96.95±9.67) h，显著长于革兰阴性杆菌的平均阳性报警时间(15.43±16.56) h、革兰阳性球菌的平均阳性报警时间(24.22±21.47) h和酵母样真菌的平均阳性报警时间(50.60±26.48) h （t’值分别为15.22、10.46、5.17，均P＜0.01）。结论布鲁菌在BACTECTM9120全自动血培养仪中的阳性报警时间明显长于其他病原菌；来源于骨髓标本的布鲁菌阳性报警时间明显短于来源于血液标本菌株。

Positive alarming time of Brucella detected by automated blood culture system and comparison with other pathogens

ObjectiveTo explore the laboratory culture features of Brucella,and analyze positive alarming time （PAT） of Brucella detected by BACTECTM9120 automated blood culture system．MethodsPAT of 76 Brucella isolates from clinical specimens were analyzed statistically, and compared with PAT of other pathogens.ResultsOf 76 Brucella isolates, 66（86.85%） were from blood specimen, 7（9.22%） were from bone marrow, and isolate from pus, cerebrospinal fluid and joint fluid was 1 （1.31%） for each. PAT of 76 Brucella was 72-120 h, the mean PAT of Brucella from bone marrow specimen was （84.51±8.86） h, which was significantly shorter than （98.27±10.32） h of blood specimen（t=3.39，P＜0.01）. The mean APT of Brucella from all specimens was （96.95±9.67） h, which was significantly longer than that of gram negative bacilli （［15.43±16.56］ h）, gram positive cocci （［24.22±21.47］ h） and yeast（［50.60±26.48］ h） （t＇=15.22,10.46,5.17，respectively, all P＜0.01）. ConclusionPAT of Brucella detected by BACTECTM9120 automated blood culture system is significantly longer than other pathogens, PAT of Brucella from bone marrow specimen is significantly shorter than that from blood specimen.