肺癌细胞株对6种抗癌药物敏感性测定

目的　探讨肺癌细胞株抗癌药物的敏感性。方法　采用体外MTT比色分析法对 10株肺癌细胞进行NVB、GEM、TAX、SN - 38、CDDP、5 -Fu 6种抗癌药物的体外敏感试验。结果　 ( 1) 6种抗癌药物对SCLC的显效顺序依次为GEM、NVB、CDDP、TAX、5 -Fu、SN - 38,4株SCLC的敏感性顺序依次为Lu135、N2 31、H6 9、SBC - 3;( 2 ) 6种抗癌药物对NSCLC的显效顺序依次为 5 -Fu、NVB、GEM、CDDP、TAX ,对SN - 38均为耐药 ,3种 6株NSCLC的敏感性顺序依次为Lu 6 5、PC 7、LK - 2、PC 9、PC 14、A 5 49;( 3)SCLC株对 6种抗癌药物敏感性优于NSCLC ,6种抗癌药物对 4株SCLC的总有效率为 87 5 % ,敏感为 6 2 5 % ,较敏感为 2 5 % ,耐药为12 5 % ;对 6株NSCLC的总有效率为 5 2 78% ,敏感为 16 6 7% ,较敏感为 36 11% ,耐药为 4 4 4 4%。结论　 10株肺癌细胞株对 5 -Fu最敏感 ,GEM、NVB、CDDP、TAX次之 ,SN - 38效果最差。 6种药物中对 5 -Fu、CDDP未表现出明显耐药性 ,其余 4种均存在不同程度的耐药性 ,表明肺癌细胞株存在自然耐药性。

Detection of Sensitivity of Lung Cancer Cell Lines to Six Types of Anticnacer Drugs

Objective To investigate the sensitivity of lung cancer cell lines to anticancer drugs. Methods The sensitivity of 10 lung cancer lines to NVB, GEM, TAX, SN-38, CDDP and 5-Fu was detected by MTT colorimetric assay in vitro. Results a. 6 types of anticancer drugs to which SCLC cell lines responded were NVB, GEM, TAX, SN-38, CDDP and 5-Fu in descending order of response. The 4 sensitive SCLC cell lines in descending order of resistance were Lu135, N231, H69 and SBC-32) six types of anticancer drugs to which NSCLC cell lines responsed were 5-Fu, NVB, GEM, TAX, CDDP and SN-38 in descending order of response. 6 cell lines of three types of NSCLC which responsed to anticancer drugs were Lu65, PC7, LK-2, PC9, PC14 and A549 in descending order of resistance. The sensitivity of SCLC cell lines to 6 types of anticancer drugs was superior to that of NSCLC cell lines. The overall response rate of 4 SCLC cell lines treated with 6 types of anticancer drugs was 87.5%. The high sensitivity rate was 62.5%. The most sensitivity rate was 25%. The resistance rate was 12.5%. The overall response rate of 6 NSCLC was 52.78%. The high sensitivity rate was 16,67%. The most sensitivity rate was 36.11%. The resistance rate was 44.44%. Conclusion Ten lung cancer cell lines were high in sensitivity to 5-Fu, were median sensitive to GEM, NVB, CDDP, TAX, were not sensitive to SN-38. The lung cancer cell lines did not show resistance to 5-Fu and CDDP. But they showed different resistance to the other 4 types of anticancer drugs. We concluded that lung cancer cell lines had intrinsic resistance to anticancer drugs.