双歧杆菌完整肽聚糖对重组乙肝表面抗原细胞免疫应答的影响

目的:探讨双歧杆菌完整肽聚糖(BWPG)对重组乙肝表面抗原(rHBsAg)诱导细胞免疫应答的影响。方法:以BWPG作为rHBsAg的佐剂免疫BALB/c小鼠,观察免疫小鼠的生长状态及中枢淋巴器官的发育情况,检测NK细胞和CTL杀伤活性的变化,脾淋巴细胞的增殖和细胞因子的产生。结果:免疫小鼠未出现可见的毒副反应,胸腺和脾脏组织呈增生状态,脾脏NK细胞和CTL的杀伤活性增强,脾淋巴细胞体外增殖活跃,细胞因子产生增多。结论:BWPG能促进中枢淋巴器官增生,增强机体对rHBsAg的细胞免疫应答。

Influence of bifidobacterial whole peptidoglycan on cellular immune response induced by recombinant HBsAg

AIM: To explore the effect of bifidobacterial whole peptidoglycan (BWPG) on cellular immune response to recombinant hepatitis B surface antigen (rHBsAg). METHODS: BWPG was used as an adjuvant of rHBsAg to immunize BALB/c mice. The growth state and development of central lymphoid organ of immunized mice were observed. Then the killing activities of NK cells and CTLs, the proliferation and cytokine production of splenic lymphocytes were examined. RESULTS: After immunization with BWPG, no visible side effect was observed in immunized mice. The thymus and spleen tissues assumed hyperplasia appearance. The killing activities of NK cells and CTLs in spleen were enhanced. The proliferation of splenic lymphocytes was active and cytokine production was increased. CONCLUSION: BWPG can promote hyperplasia of central lymphoid organ and enhance the cellular immune response to rHBsAg.