Mutations in the genome of porcine reproductive and respiratory syndrome virus responsible for the attenuation phenotype |
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| Authors: | R Allende G F Kutish W Laegreid Z Lu T L Lewis D L Rock J Friesen J A Galeota A R Doster F A Osorio |
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| Institution: | (1) Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A. , US;(2) Plum Island Animal Disease Center, USDA/ARS, Greenport, New York, U.S.A. , US;(3) U.S. Meat Animal Research Center, USDA/ARS, Clay Center, Nebraska, U.S.A. , US |
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| Abstract: | Summary. Although live-attenuated vaccines have been used for some time to control clinical symptoms of the porcine reproductive and
respiratory syndrome (PRRS), the molecular bases for the attenuated phenotype remain unclear. We had previously determined
the genomic sequence of the pathogenic PRRSV 16244B. Limited comparisons of the structural protein coding sequence of an attenuated
vaccine strain have shown 98% homology to the pathogenic 16244B. Here we have confirmed the attenuated phenotype and determined
the genomic sequence of that attenuated PRRSV vaccine and compared it to its parental VR-2332 and the 16244B strains. The
attenuated vaccine sequence was colinear with that of the strain 16244B sequence containing no gaps and 212 substitutions
over 15,374 determined nucleotide sequence. We identified nine amino acid changes distributed in Nsp1β, Nsp2, Nsp10, ORF2,
ORF3, ORF5 and ORF6. These changes may provide the molecular bases for the observed attenuated phenotype.
Received August 28, 1999 Accepted December 16, 1999 |
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