国产试剂一步法与两步法检测抗-HCV结果比较

目的：比较一步法与两步法抗-HCVELISA检测试剂的特异性及灵敏度。方法：分别采用两个不同厂家的一步法及两步法ELISA试剂，对本中心标本进行试验，对比分析两种试剂的血液报废率及检测结果。结果：使用两步法ELISA检测试剂后，血液报废率相对于一步法检测试剂略有下降，可疑阳性标本数明显下降。但灰区和可疑弱阳性标本数两个厂家间有较大差异。不同的两步法检测试剂符合率仍有待提高。结论：两步法抗一HCVELISA检测试剂优于一步法，但特异性及灵敏度仍有待加强。

Comparison of one step and two step anti-HCV domestic detection reagents

Objective：To compare the specificity and sensitivity of the one step and two step anti-HCV ELISA rea- gents. Method：Specimens of the center were respectively detected using two different manufacturer of one-step and two- step ELISA reagents. The detection results and blood scrap rate were compared. Result; Mter using two-step ELISA de- tection reagent, the blood scrap rate fell slightly compared with one-step detection reagent, the suspicious positive speci- mens decreased obviously. But the gray area specimens and suspicious weakly positive samples had great difference be- tween the two manufacturers. Two step detection reagents coincidence rate remained to be improved. Conclusion：Two-step anti-HCV ELISA detection reagents would be superior to the one step method,but the specificity and sensitivity remain to be strengthened.