丁基苯酞对创伤性脑损伤模型大鼠神经功能的改善作用

目的 探讨丁基苯酞(NBP)对创伤性脑损伤(TBI)模型大鼠神经功能的改善作用,以及对IRE1/XBP1信号通路的影响.方法 将60只大鼠分为假手术组、模型组,以及NBP高、中、低剂量组(40,20,10mg/kg),各12只.采用改良Feeney自由落体撞击法复制大鼠TBI模型.建模后,各组大鼠腹腔注射相应药物,每天...

Effect of Dl-3-n-Butylphthalide on the Improvement of Neurological Function in Rats with Traumatic Brain Injury

Objective To investigate the effect of dl-3-n-butylphthalide(NBP)on the improvement of neurological function in rats with traumatic brain injury(TBI)and the effect on IRE1/XBP1 signaling pathway.Methods A total of 60 rats were divided into sham operation group,model group,NBP high-dose,medium-dose and low-dose groups(40,20,10 mg/kg),12 rats in each group.TBI model of rat was established by the modified Feeney’s free falling method.After modeling,rats in each group were intraperitoneally injected with corresponding drugs once a day for 7 d.The neurological deficit of rats was evaluated,the brain water content was measured,the apoptosis of brain cells was evaluated by the TUNEL method and the apoptosis index was calculated.The levels of malondialdehyde(MDA)and superoxide dismutase(SOD)in brain homogenate were measured by enzyme-linked immunosorbent assay(ELISA).The levels of protein kinase C receptor 1(RACK1),phosphorylated IRE1(p-IRE1),X-box binding protein 1(XBP1)and glucose-regulated protein 78(GRP78)were determined by polymerase chain reaction(PCR).Results Compared with those in the model group,the neurological deficit score,apoptosis index,the levels of MDA,p-IRE1,XBP1 and GRP78 were significantly decreased,while the levels of SOD and RACK1 were significantly increased,and the brain water content was significantly increased in NBP high-dose,medium-dose and low-dose groups(P<0.05).Conclusion NBP has an improvement effect on the neurological function of TBI rats,and its mechanism may be related to its inhibition of IRE1/XBP1 signaling pathway.