小型猪内翻膝内侧半月板的基因表达谱分析

背景:内翻型膝关节骨性关节炎常合并有明显的内侧半月板病变,然而,其发生机制尚不明确.目的:寻找内翻膝继发内侧半月板病变的分子机制.方法:切除7只成年五指山小型猪右侧后肢前交叉韧带及外侧副韧带,造成内翻膝模型(实验侧),而左侧后肢行假手术(对照侧).造模后26周时取内侧半月板,行基因芯片分析.实验中的所有程序均于2017...

Gene expression profiling of the medial meniscus in a minipig model of varus knee

BACKGROUND: Varus-type osteoarthritis is often accompanied by obvious medial meniscus lesions. However, the underlying mechanism of its occurrence isyet unclear.OBJECTIVE: To explore the molecular mechanism of medial meniscus lesions secondary to varus knees.METHODS: The anterior cruciate ligament and lateral collateral ligament of the right rear limbs of seven Wuzhishan minipigs were resected to establish a varusknee model (experimental side), while the left hind legs were subjected to sham operation (control side). Medial meniscus samples were taken for a gene chipanalysis at 26 weeks after modeling. An ethic approval was obtained from the Medical Ethics Committee of Hainan General Hospital with an approval No. Med-Eth-Re[2020]5 on December 5, 2017.RESULTS AND CONCLUSION: The samples in the two groups showed significant differences in the gene expression. A total of 893 differentially expressed geneswere found, in which 537 were upregulated and 356 were downregulated. The significantly affected biological processes included sex determination, interstitialmorphogenesis, and regulation of nitric oxide biosynthetic process. The significantly affected cellular components included intrinsic component of membrane,integral component of plasma membrane, endoplasmic reticulum membrane, and epinuclear membrane-endoplasmic reticulum network. The significantlyaffected molecular functions included transition metal ion binding and iron ion binding. The significantly affected pathways included type II diabetes mellitus,inflammatory mediator regulation of TRP channels, and AMPK signaling pathway. These findings suggest significant changes in gene expression of varus kneemeniscus, and these differentially expressed genes may reveal the mechanism of medial meniscus lesions secondary to varus knee, providing a potential targetfor its treatment and early diagnosis.