白藜芦醇诱导佐剂性关节炎大鼠滑膜细胞凋亡

目的：探讨白藜芦醇对佐剂性关节炎( AA)大鼠滑膜细胞增殖和凋亡的影响。方法0．1 ml弗氏完全佐剂注射SD大鼠左后足跖皮内复制AA模型,28 d后,采用组织块培养法分离、培养大鼠踝关节滑膜细胞, CCK-8法检测白藜芦醇对滑膜细胞增殖的影响；Hoechst 33258荧光染色、琼脂糖凝胶电泳观察滑膜细胞凋亡情况。结果 CCK-8法表明白藜芦醇可以抑制滑膜细胞增殖,并且呈剂量依赖性趋势；高浓度白藜芦醇(20、50μmol/L )能诱导滑膜细胞凋亡, Ho-echst 33258染色观察到细胞核出现凋亡小体,电泳呈现出阶梯状条带。结论白藜芦醇可能会通过抑制AA大鼠滑膜细胞的增生,促进滑膜细胞的凋亡而达到治疗类风湿性关节炎的作用。

Resveratrol-induced apoptosis of synovial cell in adjuvant arthritis rats

Objective To investigate the effect of resveratrol on synovial cell proliferation and apoptosis in adjuvant arthritis ( AA) rats. Methods 0. 1 ml of freund′s complete adjuvant was injected into the left foot plantar skin of SD rats to copy the AA model. After 28 days, rats ankle synovial cells were isolated and cultured with tissue pieces culture method, the effect of resveratrol on synovial cell proliferation was detected by CCK-8 method;synovial cells apoptosis was observed by Hoechst 33258 fluorescent dye and agarose gel electrophoresis. Results CCK-8 method showed that resveratrol could inhibit the synovial cell proliferation, and showed a trend of dose dependent. High dose of resveratrol (20, 50 μmol/L) could induce the apoptosis of synovial cells, apoptotic body of synovial cells nucleus appeared with Hoechst 33258 dyeing, electrophoresis showed ladder-like stripe. Conclusion Resveratrol can inhibit AA rats synovial cell proliferation and promote apoptosis of synovial cell which improves the treatment of rheumatoid arthritis.