克劳斯法测定纤维蛋白原及其影响因素

目的 探讨克劳斯法的影响因素，以推荐在我国临床实验室应用克劳斯法测定纤维蛋白原。方法 分别采用进口试剂盒和国产试剂，建立各自的纤维蛋白原标准曲线，并通过对标准品的测定进行比较，探讨缓冲液ｐＨ、凝血酶浓度和测定温度对测定结果的影响。结果 应用国产试剂，建立的克劳斯法，重复性良好，日内ＣＶ＝０．０３９１，日间ＣＶ＝０．０４９７。对标准品分别用进口试剂盒和国产试剂进行测定，相对误差分别为＋３．１７％、＋４．７６％和－６．０３％。对１份冻干血浆测定，两者结果相近。结论 完全要用国产试剂代替价格昂贵的进口试剂盒，有利于克功斯法在国内的推广应用。

The vonClauss method for fibrinogen assay and its influential factors

Objectives To study the influential factors of the von Clauss method and to promote the method for fibrinogen assay in clinical laboratories in China. MethodsKits of foreign and domestic products were used. Calibration curves for fibrinogen determination were established, and calibration plasma was used for comparison. Effects of pH value, thrombin activity, and temperature on the measurement were studied.Results The reproducibility of the von Clauss method using domestic thrombin and related reagents was excellent, the within day CV being 0.039 1, and the between day CV 0.049 7. The percentage variations of calibration plasma determination, with the foreign fibrinogen assay kits using domestic reagents, were 3.17% , 4.76%, and -6.03%, respectively. Determination of a lyophilized plasma using the two sources reagents showed similar results.Conclusions Thrombin and relevant reagents produced in China could be used as a substitute for kits purchased abroad, which would promote the use of von Clauss method in clinical laboratories of China.