黄连、吴茱萸不同配比对大鼠热耐受能力的影响研究

目的 观察黄连与吴茱萸不同配伍比例（黄连-吴茱萸分别为6：1、2：1、1：1、1：6）在热环境下对大鼠肛温、生存时间和死亡率的影响,确定其对增强大鼠热耐受能力的差异.方法 将SD大鼠随机分为6组,空白对照组（A组）给生理盐水,阳性对照组（B组）给藿香正气水,实验组（C、D、E、F组）分别给黄连、吴茱萸药对各配伍比例提取物.6组同时放入温度为41℃,相对湿度为70%的人工热室中受热至死.热暴露前测各鼠肛温1次,热暴露1 h后再测1次肛温,并记录各鼠死亡时间.结果 B组和C、D、E、F组均能不同程度降低大鼠肛温、死亡率,延长存活时间,其中以D组效果最为显著.结论 黄连-吴茱萸2：1能显著增强大鼠热环境的耐受能力,抗中暑作用最为明显.

Effect of compatibility of Coptis chinensis Franch and Evodia rutaecarpa (Juss) Benth on heat tolerance

Objective To observe the effect of compatibility in Coptis chinensis Franch and Evodia rutaecarpa （Juss） Benth （6 ： 1, 2 ： 1, 1 ： 1, 1 ： 6） on anus temperature, survival time, and mortality in thermal environment in rats and the difference in enhancing the heat tolerance. Methods SD rats were randomly divided into 6 groups： the blank group was treated with saline water, the positive control group with the agastache rugosaq healthy water, while the experimental group C, D, E, and F with various compatibility proportions of extracts in Coptis chinensis Franch and Evodia rutaecarpa （Juss） Benth. Then the rats were exposed to heat at 41 ℃ in a heat chamber with relative humidity of 70% till death. The rectal temperature was measured before and 1 h after the heat exposure, and the time when death occurred were recorded. Results Compared with the blank group, group B, C, D, E and F had lower rectal temperature and prolonged survival time with decreased mortality. Group D had better action than the others. Conclusion Coptis chinensis Franch and Evodia rutaecarpa （Juss） Benth （2 ： 1） can significantly enhance the thermal environment of tolerance, which has the most obvious heat resistant function.