| 用硝酸镧示踪法观察渗透性血脑屏障开放后的超微结构变化 |
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| 引用本文： | 王振宇,王毅,易声禹,李学荣.用硝酸镧示踪法观察渗透性血脑屏障开放后的超微结构变化[J].第四军医大学学报,1986(4). |
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| 作者姓名： | 王振宇 王毅 易声禹 李学荣 |
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| 作者单位： | 第四军医大学附属一院神经外科
(王振宇，王毅，易声禹)，第四军医大学电镜室(李学荣) |
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| 摘 要： | 用兔颈内动脉注入1.4M甘露醇法、制作了渗透性血脑屏障开放的模型。选硝酸镧作示踪剂,用血管灌注法和块染法对血脑屏障开放后的超微结构变化进行了观察。电镜检查表明镧可由血管内通过内皮细胞间的紧密连接,达内皮下层和细胞外间隙,或从血管外进入血管内。实验提示:渗透性血脑屏障开放的形态学基础是血管内皮细胞间的紧密连接开闭的可逆性改变。镧血管灌注法的示踪效果优于镧的块染法。
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| 关 键 词： | 血脑屏障 硝酸镧 甘露醇 超微结构 示踪法 动物模型 |
Ultrastructural Observation on Osmotic Opening of Blood-Brain Barrier Under Carotid Infusion of Mannitol and lanthanum Nitrate Tracer |
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| Wang Zhenyu,et al.Ultrastructural Observation on Osmotic Opening of Blood-Brain Barrier Under Carotid Infusion of Mannitol and lanthanum Nitrate Tracer[J].Journal of the Fourth Military Medical University,1986(4). |
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| Authors: | Wang Zhenyu |
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| Abstract: | A model of osmotic opening of Blood-Brain Barrier (BBB) was successfully achieved by infusion of 1.4 M mannitol into the internal carotid artery of rabbits. We selected lanthanum nitrate as a tracer. Ultrastructural changes of osmotic opening of BBB were observed through injection of lanthanum nitrate into blood vessels or impreguation of the small block of brain tissue in lanthanum. Electron microscopy showed the opening of endothelial tight junctions through which lanthanum nitrate passed from blood to subendothelial layers and extracellular space of the brain, or from extravessels to intravessels. The data suggest that the morphological basis of osmotic opening of BBB is the reversible width of tight junctions between endothelial cells, and that lanthanum nitrate infusion has an advantage over the block imprgnation. |
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| Keywords: | blood-brain barrier lanthanum nitrate mannitol ultrostructure tracing animal model |
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