| 羌活不同地理种群ITS序列变异及系统发生分析 |
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| 引用本文： | 杨路存,刘何春,周学丽,徐文华,周国英.羌活不同地理种群ITS序列变异及系统发生分析[J].中国中药杂志,2015,40(19):3748-3753. |
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| 作者姓名： | 杨路存 刘何春 周学丽 徐文华 周国英 |
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| 作者单位： | 中国科学院 西北高原生物研究所, 青海 西宁 810008;中国科学院 藏药研究重点实验室, 青海 西宁 810008,中国科学院 西北高原生物研究所, 青海 西宁 810008;中国科学院大学, 北京 100049,青海省铁卜加草原改良试验站, 青海 西宁 810008,中国科学院 西北高原生物研究所, 青海 西宁 810008;中国科学院 藏药研究重点实验室, 青海 西宁 810008,中国科学院 西北高原生物研究所, 青海 西宁 810008;中国科学院 藏药研究重点实验室, 青海 西宁 810008 |
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| 基金项目： | 青海省青年基金项目(2013-Z-942Q) |
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| 摘 要： | 利用核糖体rRNA基因内转录间隔区(ITS)序列,对我国羌活31个野生群体的遗传结构进行了分析。经PCR扩增测序,获得长度为634~635 bp的ITS核苷酸序列,其平均G+C量(57.8%)显著高于A+T量。在羌活31个居群402条序列中共检测到31个变异位点,多态位点比例为4.88%,其中,简约信息位点为12个,共有31种单倍型。AMOVA分析结果显示:羌活大部分的遗传变异发生在居群间(57%)。以宽叶羌活为外群构建的NJ树显示:31个单倍型并没有按地理分布形成明显的族群,各地理单元中的单倍型相互混杂,没有明显的地理分化模式。
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| 关 键 词： | 羌活 ITS 序列分析 遗传结构 |
| 收稿时间： | 2015/3/31 0:00:00 |
ITS sequences variation and phylogenetic analysis on 31 geographical populations of Notopterygium incisum |
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| YANG Lu-cun,LIU He-chun,ZHOU Xue-li,XU Wen-hua and ZHOU Guo-ying.ITS sequences variation and phylogenetic analysis on 31 geographical populations of Notopterygium incisum[J].China Journal of Chinese Materia Medica,2015,40(19):3748-3753. |
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| Authors: | YANG Lu-cun LIU He-chun ZHOU Xue-li XU Wen-hua and ZHOU Guo-ying |
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| Institution: | Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China;Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences, Xining 810008, China,Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China;University of Chinese Academy of Sciences, Beijing 100049, China,Tiebujia Grassland Improvement Experiment Station, Xining 810008, China,Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China;Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences, Xining 810008, China and Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China;Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences, Xining 810008, China |
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| Abstract: | In this study, 31 Notopterygium incisum populations were analyzed using ITS sequences to investigate the genetic structure.The results showed that: the ITS region ranged in size from 634 to 635 bp and base composition was with high G+C content of 57.8%. Thirty-one polymorphic sites were detected from 402 sequences of 31 populations of N. incisum, and the proportion of polymorphic sites was 4.88%, in which parsimony informative sites were up to 12. And 31 haplotypes were identified based on these polymorphic sites. Molecular variance analysis (AMOVA) indicated that high genetic differentiation (57%) existed among population, and gene flow was low (Nm=0.38) among populations. Phylogenetic relationships of 31 haplotypes were analyzed using NJ method with N. forbesii as an out-group. Phylogenetic analysis showed that 31 haplotypes from different populations mixed together and did not form distinct geographically separated clades. |
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| Keywords: | Notopterygium incisum ITS sequence analysis genetic structure |
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