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| RP-HPLC测定环孢菌素A凝胶剂中环孢菌素A的含量 | |
| 引用本文： | 徐崇范,倪海镜,卢海霞,韩铭. RP-HPLC测定环孢菌素A凝胶剂中环孢菌素A的含量[J]. 沈阳药科大学学报, 2002, 19(6): 427-429 |
| 作者姓名： | 徐崇范 倪海镜 卢海霞 韩铭 |
| 作者单位： | 1. 吉林省药品检验所,吉林,长春,130062 2. 吉林大学中日联谊医院,吉林,长春,130021 3. 长春一汽总医院,吉林,长春,130011 |
| 摘 要： | 目的测定环孢菌素A凝胶剂中环孢菌素A的含量。方法反相高效液相色谱法 ,采用Kro masilC8色谱柱 ,甲醇∶水 (90∶10 ,V∶V)为流动相 ,2 14nm为检测波长 ,在室温条件下对环孢菌素A凝胶剂进行含量测定。结果环孢菌素A与其他组分能较好的分离 ,在 1 4 14～ 7 2 34μmol/L内 ,环孢菌素A峰面积与浓度呈良好的线性关系 (r =0 9999) ;样品平均回收率为 99 98% (RSD =1 1% )。结论该方法可有效地控制产品质量 |
| 关 键 词： | 环孢菌素A 含量 反相高效液相色谱法 |
| 文章编号： | 1006-2858(2002)06-0427-03 |
| 修稿时间： | 2002-05-14 |
Determination of the content of the cyclosporine A in the cyclosporine A gel by the reversed-phase HPLC |
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| XU Chong fan,NI Hai jing,LU Hai xia,HAN Ming. Determination of the content of the cyclosporine A in the cyclosporine A gel by the reversed-phase HPLC[J]. Journal of Shenyang Pharmaceutical University, 2002, 19(6): 427-429 | |
| Authors: | XU Chong fan NI Hai jing LU Hai xia HAN Ming |
| Abstract: | Objective To develop a RP HPLC method to determine the content of cyclosporine A in the cyclosporine A gel. Methods The kromasil C 8 column was used.The mobile phase consisted of methanol and water (90∶10, V∶V) ,the peaks were detected at 214 nm .The column temperature was the room temperature.Results Cyclosporine A could be isolated from other components.The calibration curves were liner in the range of 1 414 μmol/L ～ 7 234 μmol/L ( r =0 999 9 ) for CsA. The average recovery was 99 98% with RSD=1 1% ( n =5). Conclusions The method can evaluate well and the true quality of the Cyclosporine A gel. |
| Keywords: | cyclosporine A content reversed phase HPLC |
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