新疆哈萨克族食管癌组织中乳头瘤病毒16型E6E7基因的研究

为了解HPV16感染后E6E7基因变化在哈萨克族食管癌发病中的作用 ,以及HPV16E6E7基因与食管癌病理组织分级的关系 ,采用聚合酶链 (PCR)技术 ,检测 82例食管癌及癌旁正常食管粘膜组织中HPV 16E6E7基因差别。食管癌、癌旁正常粘膜组HPV16E6E7基因的检出率分别为 3 4. 15 % ( 14 /4 1)和 19. 5 1% ( 8/4 1) ,63 . 41% ( 2 6/4 1)和48. 78% ( 2 0 /4 1) ,差别均无显著性 (P >0 . 0 5 )。然而 ,在食管癌组织的高分化组、中低分化组中HPV16E6E7的检出率分别为 7. 69% ( 1/13 )和 46. 43 % ( 13 /2 8) ,3 8. 46% ( 5 /13 )和 75 % ( 2 1/2 8) ,差别均有统计学意义 (P <0 .0 5 )。提示HPV16E6E7基因与哈萨克族食管癌的发生及癌组织分级密切相关。

Study on HPV 16 E6 E7 in Tissue of Kazak's Esophageal Cancer in Xinjiang

To examine the association between genes of HPV16 E6 E7 and susceptibility to Kazak population esophageal cancer, a polymerase chain reaction method was used to detect HPV16E6 E7 genes in genomic DNA isolated from surgically removed or biopsied esophageal tissues from cases with cancer (n=41), cases with adjacent normal controls (n=41) from a high-risk population, Xinjiang, China. Results showed that the positive rate of the HPV16E6 and E7 genes in cancer cases (34.15% and 63.41%) was not statistically significant differences from that in controls (19.51% and 48.78%). However, the positive rate of HPV16E6 and E7 genes in cases with well-differentiated cancer(7.69% and 38.46%) showed a significant decrease compared to that in poor-differentiated cancer (46.43% and 75%)(P<0.05). The results suggest that HPV16E6 E7 genes act as risk factor in the development of poor-differentiated esophageal cancer in Kazak population.