| 胶质细胞源性神经营养因子基因转染雪旺氏细胞的实验研究 |
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| 引用本文： | 杜文彦,王景宏,刘钦毅,郑长军. 胶质细胞源性神经营养因子基因转染雪旺氏细胞的实验研究[J]. 中国实验诊断学, 2010, 14(1): 20-22 |
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| 作者姓名： | 杜文彦 王景宏 刘钦毅 郑长军 |
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| 作者单位： | 1. 佳木斯大学附属第一医院,骨科,黑龙江,佳木斯154002
2. 吉林大学第二医院,骨科,吉林,长春130041 |
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| 基金项目： | 吉林省科技厅资助课题;课题 |
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| 摘 要： | 目的应用胶质细胞源性神经营养因子(GDNF)基因转染雪旺氏细胞,观察GDNF基因在雪旺氏细胞内的表达效果,以及对雪旺氏细胞的营养作用。方法将雪旺氏细胞分为空白对照组、载体组、空载体组,通过阳离子脂质体介导,将GDNF基因转染雪旺氏细胞。用细胞增殖实验(MTT)、免疫组化、流式细胞仪观察在24h、48h、72h各组转染后对细胞的营养作用。结果在载体组细胞的生长状况较其他两组增殖良好。免疫组化可见GDNF在载体组细胞内呈现高表达。流式细胞仪观察基因转染后对雪旺氏细胞有明显的营养作用。结论GDNF基因转染雪旺氏细胞后,在细胞内呈现高表达,并对细胞有明显的营养作用。
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| 关 键 词： | 雪旺氏细胞 胶质神经源性神经营养因子（GDNF）基因 转染 |
The Experimental Study of Glial Cell Line-derived Neurotrophic Factor Gene Transfection to Schwann Cells |
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| DU Wen-yan,WANG Jing-hong,LIU Qin-yi,et al.. The Experimental Study of Glial Cell Line-derived Neurotrophic Factor Gene Transfection to Schwann Cells[J]. Chinese Journal of Laboratory Diagnosis, 2010, 14(1): 20-22 |
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| Authors: | DU Wen-yan WANG Jing-hong LIU Qin-yi et al. |
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| Affiliation: | DU Wen-yan,WANG Jing-hong,LIU Qin-yi,et al.(The Orthopedics Department of the First Hospital of Jiamusi University,Jiamusi 154002,China) |
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| Abstract: | Objective To observe the expression of GDNF and the role of nutrition and protection in Schwann cells through GDNF gene's transfetion to Schwann cells.Methods The Schwann cells were divided into control group,empty vector group,vector group.The GDNF gene was transfected into Schwann cells through cationic liposome mediattion.The nutritional function and expression effect were examined though MTT,immunohistochemistry,flow cytometry at 24 h,48 h,72 h after transfection of each group.Results The cells in vecto... |
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| Keywords: | Schwann cells Glial-derived neurotrophic factor transfection |
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