A Novel Approach to Increase the Stability of Liposomal Containers via In Prep Coating by Poly[N‐(2‐Hydroxypropyl)Methacrylamide] with Covalently Attached Cholesterol Groups |
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| Authors: | Olga V. Zaborova Sergey K. Filippov Petr Chytil Lubomir Kováčik Karel Ulbrich Alexander A. Yaroslavov Tomaš Etrych |
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| Affiliation: | 1. Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia;2. Institute of Macromolecular Chemistry, Czech Academy of Sciences, Prague 6, Czech Republic;3. Center for Cellular Imaging and NanoAnalytics (C‐CINA) Biozentrum, University of Basel, Basel, Switzerland |
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| Abstract: | Highly stable liposomes are developed by coating phosphatidylcholine liposomes with amphiphilic N‐(2‐hydroxypropyl)methacrylamide copolymer. Two approaches in the preparation of coated liposomes are employed: the copolymer is added during (“in prep”) or after (“ex post”) the liposome formation. The influence of polymer concentration and coating method is evaluated using the cryogenic transmission electron microscopy, dynamic light scattering, and small‐angle X‐ray scattering techniques. The in prep modification significantly increases, up to four weeks, the stability of liposomes against aggregation and makes the liposomal membrane nonpermeable toward an inorganic salt. Such enhanced longevity is attributed to the different structure of in prep coated liposomal membranes. |
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| Keywords: | copolymers enhanced longevity liposomes N‐(2‐hydroxypropyl)methacrylamide SAXS |
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