Hydrolytic Stability of Methacrylamide and Methacrylate in Gelatin Methacryloyl and Decoupling of Gelatin Methacrylamide from Gelatin Methacryloyl through Hydrolysis |
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| Authors: | Jing Zheng Mengxiang Zhu Gaia Ferracci Nam‐Joon Cho Bae Hoon Lee |
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| Affiliation: | 1. School of Ophthalmology and Optometry, Eye Hospital, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, China;2. Wenzhou Institute of Biomaterials and Engineering, Wenzhou, Zhejiang, China;3. School of Materials Science and Engineering, Nanyang Technological University, Singapore;4. Engineering Research Center of Clinical Functional Materials and Diagnosis and Treatment Devices of Zhejiang Province, Wenzhou Institute of Biomaterials and Engineering, Wenzhou, Zhejiang, China |
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| Abstract: | Gelatin methacryloyl (GelMA; GM) is a promising nature‐derived photocurable material that can mimic the extracellular matrix because GelMA features tailorable mechanical properties, proteolytic degradation, and good cell adhesion. GelMA contains not only methacrylamide but also methacrylate. However, the hydrolytic stability of methacrylamide and methacrylate groups of GelMA in aqueous solutions has not been scrutinized. Here, the structural change of GelMA through hydrolysis is investigated for the first time. The structural change of hydrolyzed GelMA is quantitatively identified using colorimetric and 1H NMR methods. The methacrylate groups decompose markedly at high pH solutions, but the methacrylamide groups remain stable. Further, pure gelatin methacrylamide is successfully decoupled from GelMA for a better understanding of GelMA structure and future use for biomedical applications. |
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| Keywords: | gelatin methacrylamide gelatin methacrylate gelatin methacryloyl hydrolysis structural stability |
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