雌激素对阴道上皮细胞抗念珠菌作用的影响

  目的：研究阴道上皮细胞对白色念珠菌的生长抑制作用及雌激素对此作用的影响。 方法： 用酶消化法分别分离雌激素处理组、动情间期组和去势组小鼠阴道上皮细胞作为效应细胞，白色念珠菌芽生孢子作为靶细胞，将效靶细胞共同孵育9 h，测定白色念珠菌生长抑制率，观察其生长密度及透射电镜超微结构的改变。 结果： 白色念珠菌与阴道上皮细胞共同孵育后，生长受到抑制，生长密度降低，超微结构表现为与阴道上皮细胞靠近的念珠菌细胞壁崩解，细胞膜连续性破坏，甚至细胞器肿胀溶解。雌激素处理组与去势组和动情间期组比较，阴道上皮细胞对念珠菌的生长抑制率下降（P<0.05）。 结论： 小鼠阴道上皮细胞具有天然的抗白色念珠菌作用，雌激素降低此抑制作用。

Effect of estrogen on murine vaginal epithelial cell anti-Candida activity

AIM: To investigate the growth inhibition of Candida albicans mediated by vaginal epithelial cells and determine if estrogen affects the anti-fungal activity. METHODS: C57BL/6J mice vaginae from estrogen treatment group, diestrus group and ovariectomized group were excised, respectively. The vaginae were dissociated into single cell suspension by dispase and collagenaseⅠ. The epithelial nenriched cells were used as effector cells. Blastoconidia of C. albicans were used as target cells. After coincubation of effector with target for 9 h, the target cells growth density was observed, percent growth inhibition was calculated, and ultra-structural changes were observed. RESULTS: After coincubation of effector cells with target cells for 9 h, growth density of C. albicans was visibly reduced and it's growth activity was inhibited. Compared to ovariectomized group and diestrus group, vaginal epithelial cells from estrogen-treated mice had less ability to inhibit the growth of C. albicans (P<0.05). C. albicans incubated alone showed intact and legible ultrastructure while the Candida coincubated near the epithelial cells showed obvious changes: the cell wall ruptured, intact cytoplasmic membrane was damaged, intracellar component dissolved. CONCLUSION: The results suggest that mice vaginal epithelial cells have innate anti-Candida activity in vitro and the activity was inhibited by estrogen.