| 叶下珠指纹图谱及模式识别研究 |
| |
| 引用本文： | 吴玲芳,林琛,袁永兵,孙雪飞,朱丹,杨光辉,邵岩岩,毛鑫,张兰珍,石任兵. 叶下珠指纹图谱及模式识别研究[J]. 中草药, 2015, 46(4): 572-575 |
| |
| 作者姓名： | 吴玲芳 林琛 袁永兵 孙雪飞 朱丹 杨光辉 邵岩岩 毛鑫 张兰珍 石任兵 |
| |
| 作者单位： | 北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;天津药物研究院, 天津 300193;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102 |
| |
| 基金项目： | 国家自然科学基金资助项目(30171197);北京市自然基金项目(7073093);北京市教育委员会科技成果转化与产业化项目;北京中医药大学科研创新团队(2011-CXTD-12) |
| |
| 摘 要： | 目的建立叶下珠HPLC指纹图谱评价体系。方法采用Diamonsil C18(250 mm×4.6 mm,5μm)色谱柱,乙腈-0.2%冰醋酸水溶液为流动相梯度洗脱,检测波长270 nm,体积流量1.0 m L/min。测定9个不同产地叶下珠指纹图谱,采用中药色谱指纹图谱相似度评价系统(2004A版)计算相似度,并用对照品和液质联用技术指认色谱峰。结果建立了叶下珠指纹图谱共有模式,标定了11个共有峰,指认了其中8个主要色谱峰,用聚类分析和主成分分析对指纹图谱进行模式识别。结论将指纹图谱和模式识别结合起来对叶下珠进行质量控制是一种有效的评价方法,为叶下珠的全面质量控制提供了基础。
|
| 关 键 词： | 叶下珠 指纹图谱 模式识别 HPLC 聚类分析 |
| 收稿时间： | 2014-10-14 |
Fingerprint analysis and chemical pattern recognition for whole plant of Phyllanthus urinaria |
| |
| WU Ling-fang,LIN Chen,YUAN Yong-bing,SUN Xue-fei,ZHU Dan,YANG Guang-hui,SHAO Yan-yan,MAO Xin,ZHANG Lan-zhen and SHI Ren-bing. Fingerprint analysis and chemical pattern recognition for whole plant of Phyllanthus urinaria[J]. Chinese Traditional and Herbal Drugs, 2015, 46(4): 572-575 |
| |
| Authors: | WU Ling-fang LIN Chen YUAN Yong-bing SUN Xue-fei ZHU Dan YANG Guang-hui SHAO Yan-yan MAO Xin ZHANG Lan-zhen SHI Ren-bing |
| |
| Affiliation: | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China |
| |
| Abstract: | |
| |
| Keywords: | whole plant of Phyllanthus urinaria L. fingerprint pattern recognition HPLC cluster analysis |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中草药》浏览原始摘要信息 |
|
点击此处可从《中草药》下载全文 |
|
