铜钻药材质量标准

目的:制定瑶族药铜钻药材质量标准,为瑶族药铜钻的开发利用提供技术支持。方法:分别对铜钻药材的药材性状、显微特征进行观察;按照《中国药典》方法对11个不同产地药材中浸出物、灰分和水分进行测定;以蒲公英赛酮及蒲公英赛醇为对照品,采用薄层色谱法对其进行定性鉴别;采用高效液相色谱法测定药材中香草酸和香草醛的含量。结果:对铜钻药材的性状、显微特征进行了描述。暂定铜钻药材中总灰分不得过8%,不溶性灰分不得过3%,水浸出物不得少于4%,醇浸出物不得少于3%,水分不得过11%。TLC中蒲公英赛酮和蒲公英赛醇斑点清晰、分离度好。香草醛和香草酸的精密度、稳定性、重复性试验的RSD均<2%。结论:所建标准可用于铜钻药材的质量控制。

Quality Specification of Vines of Mappianthus iodoides

Objective: To provide scientific basis for the utilization and development of vines of Mappianthus iodoides by setting up the quality standards. Method: The pharmacognostical methods were applied. The morphological and histological characters of vines of M. iodoides were observed. The extracts, moisture, total ash, and acid-insoluble ash were determined, and the bioactive constituents were analyzed by TLC and HPLC. Result: The quality specification of vines of M. iodoides were as follows:total ash was not more than 8%;acid-insoluble ash was not more than 3%;moisture was not more than 11%, water extracts not less than 4% and alcohol extracts not less than 3%. The qualitative analysis of taraxerone and taraxerol were performed by TLC and quantitative analysis of vanillin and vanillic acid were performed by HPLC, respectively. Conclusion: The established method can be used for the quality control of vines M. iodoides.