| 液质联用技术分析次乌头碱在大鼠尿液中的代谢产物 |
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| 引用本文： | 辛杨,张滢,张哲,张伟光,吕君. 液质联用技术分析次乌头碱在大鼠尿液中的代谢产物[J]. 中国实验方剂学杂志, 2015, 21(4): 94-97 |
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| 作者姓名： | 辛杨 张滢 张哲 张伟光 吕君 |
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| 作者单位： | 齐齐哈尔大学 化学与化学工程学院, 黑龙江 齐齐哈尔 161006,齐齐哈尔大学 化学与化学工程学院, 黑龙江 齐齐哈尔 161006,齐齐哈尔大学 化学与化学工程学院, 黑龙江 齐齐哈尔 161006,齐齐哈尔大学 化学与化学工程学院, 黑龙江 齐齐哈尔 161006,齐齐哈尔大学 化学与化学工程学院, 黑龙江 齐齐哈尔 161006 |
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| 基金项目： | 黑龙江省教育厅科技研究项目(12541874) |
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| 摘 要： | 目的:检测次乌头碱在大鼠体内的代谢产物及代谢途径。方法:采用液-液萃取法对灌胃给予次乌头碱的大鼠尿液样品进行纯化,利用高效液相色谱-离子阱串联质谱检测纯化样品,根据各保留时间对应化合物的多级串联质谱数据,并与文献对照,推断次乌头碱在大鼠尿液中代谢产物的结构。结果:与空白组大鼠尿液相比,在次乌头碱给药大鼠的尿液中共检测到4个乌头类生物碱,分别为次乌头碱原型化合物,18-O-去甲基次乌头碱,N-去甲基次乌头碱,被标识为羟基化的代谢物(结构有待确证)。结论:次乌头碱在大鼠体内可能发生了去甲基、羟基化代谢反应,为该成分的药效作用研究提供参考。
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| 关 键 词： | 次乌头碱 代谢产物 高效液相色谱-质谱联用 代谢途径 |
| 收稿时间： | 2014-12-06 |
Analysis on Metabolites of Hypaconitine in Rats' Urine by LC-MSn |
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| XIN Yang,ZHANG Ying,ZHANG Zhe,ZHANG Wei-guang and LYU Jun. Analysis on Metabolites of Hypaconitine in Rats' Urine by LC-MSn[J]. China Journal of Experimental Traditional Medical Formulae, 2015, 21(4): 94-97 |
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| Authors: | XIN Yang ZHANG Ying ZHANG Zhe ZHANG Wei-guang LYU Jun |
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| Affiliation: | College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China,College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China,College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China,College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China and College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China |
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| Abstract: | Objective: To analyze metabolites of hypaconitine in rats' urine. Method: Urine samples of rats were purified by liquid-liquid extraction,they were detected by liquid chromatography coupled with ion trap mass spectrometry.HPLC was performed by applying gradient elution with mobile phase composed of buffer of 2% ammonia combined with 0.2% acetic acid (A)-methanol blended with acetonitrile of equal volume (B)-methanol(C). Result: Prototype (M1) and three metabolites (M2, M3, M4) of hypaconitine were detected,M2 was identified as 18-O-demethylhypaconitine,M3 was identified as N-demethylhypaconitine,M4 was identified as a hydroxylated metabolite. Conclusion: Hypaconitine may occur demethylation,hydroxylation metabolic reactions,which could provide a reference for further research on pharmacodynamics of hypaconitine. |
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| Keywords: | hypaconitine metabolites liquid chromatography-ion trap mass spectrometry metabolic pathways |
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