阴离子交换树脂纯化安息香总香脂酸的工艺优选

目的:优选阴离子交换树脂纯化安息香总香脂酸的工艺参数。方法:采用UV测定总香脂酸含量,检测波长223 nm。以总香脂酸的吸附量和洗脱率为考察指标,通过静态吸附-洗脱试验考察717,D201,D301型阴离子交换树脂对安息香总香脂酸的纯化效果,筛选大孔树脂型号,通过单因素试验优选纯化工艺参数。结果:717型阴离子交换树脂交换能力明显强于其他2种树脂,最佳工艺条件为上样液质量浓度0.02 g·m L-1,上样液p H 12.0,上样量8 BV,上样液吸附流速2 BV·h-1,加水量3 BV,加稀盐酸1 BV洗脱,加95%乙醇5 BV洗脱,洗脱流速1 BV·h-1。总香脂酸纯度达73.49%,较纯化前26.47%增加了2.78倍。结论:717型强碱性阴离子交换树脂对安息香总香脂酸的纯化效果较好,优选的纯化工艺稳定可行。

Optimization of Purification Process of Total Balsamic Acids from Benzoinum by Anion Exchange Resin

Objective: To optimize technological parameters of purification process of total balsamic acids from Benzoinum by anion exchange resin. Method: UV was adopted to determine the content of total balsamic acids with detection wavelength at 223 nm.With adsorption capacity and eluting ratio of total balsamic acids as indexes,three types(717,D211 and D301) of anion exchange resin was seleted by static adsorption-elution test,then technological parameters of purification process was optimized by single factor test. Result: Exchange capacity of 717 anion resin was better than the other two kinds of resins,optimal technological parameters were as follows:the concentration of sample solution 0.02 g · mL^-1,pH of 12.0,sample volume of 8 BV,adsorption flow rate of 2 BV · h^-1,removed impurities with 3 BV of water and 1 BV of dilute hydrochloric acid,eluted with 5 BV of 95% ethanol,elution flow rate of 1 BV · h^-1.Under these conditions,purity of total balsamic acids could reached 73.49%,which increased 2.78 times by comparing with 26.47% before purification. Conclusion: Purification efficiency of 717 anion exchange resin for total balsamic acids from Benzoinum is good,and this optimized purification process is stable and feasible.