| 胡芦巴对Caco-2细胞胆固醇载体基因表达的影响 |
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| 引用本文： | 隋宇,王文苹.胡芦巴对Caco-2细胞胆固醇载体基因表达的影响[J].宁夏医学杂志,2010,32(12):1157-1158. |
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| 作者姓名： | 隋宇 王文苹 |
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| 作者单位： | [1]宁夏广播电视大学,宁夏银川750001 [2]宁夏医科大学,宁夏银川750004 |
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| 基金项目： | 宁夏教育厅科研资助项目 |
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| 摘 要： | 目的研究胡芦巴对小肠主要胆固醇载体ABCG5/G8、ABCA1、SR-B1和NPC1L1在Caco-2细胞基因表达的影响,探讨其降脂机制。方法将胡芦巴水溶液暴露于Caco-2细胞中24和48h作为治疗组,未加药者为对照组,从细胞中分离mRNA,进行Real-Time PCR分析,以GAPDH为内部标准测定ABCG5/G8,ABCA1和NPC1L1基因的表达。结果胡芦巴可以抑制胆固醇载体ABCG5（90%）/G8（80%）、ABCA1（70%）、SR-B1（70%）和NPC1L1（70%）表达。结论胡芦巴可抑制Caco-2细胞胆固醇载体的基因表达水平。
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| 关 键 词： | 胡芦巴 降脂机制 胆固醇载体 |
Effect of Fenugreek seed extract on cholesterol transport in Caco-2 cells |
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| Institution: | SUI Yu,et al.(Ningxia Broadcasting and TV University,Yinchuan 750001,China) |
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| Abstract: | Objective To demonstrate the effects of Fenugreek on the mRNA expression of cholesterol transporters（ATP binding cassette transporters： ABCG5/G8,ABCA1） and NPC1L1 in Caco-2 cells.Methods The expression of ABCG5/G8,ABCA1 mRNA was analyzed by the real-time PCR with an internal standard GAPDH.In order to investigate the function of cholesterol transporters,and Caco-2 cell monolayers was exposed in Fenugreek extraction solution for 24hrs and 48hrs.Results The cholesterol transporters ABCG5/G8,ABCA1,SR-B1 and NPC1L1 were inhibited by Fenugreek.Conclusion It suggests that fenugreek could suppress the function of transporters relevant to cholesterol influx by reducing the expression of ABCG5/G8,ABCA1 and NPC1L1 mRNA. |
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| Keywords: | Fenugreek The blood lipid lowering effect mechanism Cholesterol transporters |
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