Induction of simian virus 40 transplantation immunity in hamsters by gel-purified SV40 large T antigen |
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| Authors: | H Haddada N Le Goffic K Chandrasekaran C de Vaux Saint Cyr |
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| Institution: | Institut de Recherches Scientifiques sur le Cancer, Villejuif, France. |
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| Abstract: | Simian virus 40 (SV40) large T antigen and p53 cellular protein were isolated from an SV40-transformed hamster cell line by immunoprecipitation with anti-T sera and purified by sodium dodecyl sulfate-gel electrophoresis. These two protein were tested in hamsters for the presence of SV40 transplantation rejection antigenic sites by in vivo transplantation rejection assay. The large T antigen immunized the hamsters against a challenge of SV40 tumor cells and the protected animals generated cytotoxic spleen cells. Hamsters immunized with the p53 cellular protein were not protected against SV40-induced tumor but there was some delay in the appearance of tumor. |
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