RG50864对血小板衍生生长因子诱导的肺动脉平滑肌细胞DNA含量及增殖细胞核抗原表达的影响

为研究酪氨酸蛋白激酶抑制剂ＲＧ５０８６４ 对血小板衍生生长因子 （ＰＤＧＦ） 诱导的肺动脉平滑肌细胞（ＰＡＳＭＣ） ＤＮＡ 含量及增殖细胞核抗原表达的影响， 应用原代培养的小牛肺动脉平滑肌细胞， 用流式细胞仪分析ＲＧ５０８６４ 对ＰＤＧＦ诱导的ＰＡＳＭＣＤＮＡ百分含量及增殖细胞核抗原的变化。结果表明： 与对照组相比， ＲＧ５０８６４ 处理组可显著地抑制ＰＤＧＦ诱导的ＰＡＳＭＣ增殖细胞核抗原表达的荧光强度及荧光指数 （Ｐ＜ ０．０１）， 增加ＰＡＳＭＣ生长周期中的Ｇ０／Ｇ１ 期的ＤＮＡ百分含量， 抑制Ｓ期及Ｇ２Ｍ 期的ＤＮＡ 百分含量 （Ｐ＜ ０．０１）。ＲＧ５０８６４ 可显著地抑制ＰＤＧＦ诱导的ＰＡＳＭＣ增殖细胞核抗原表达，抑制ＰＡＳＭＣ周期Ｓ期及Ｇ２Ｍ 期ＤＮＡ 百分含量，阻止ＰＡＳＭＣ周期由Ｇ０／Ｇ１ 期向ＤＮＡ合成的Ｓ期及Ｇ２Ｍ 期转化

The Effect of RG50864 on DNA Percent Content and Proliferating Cells Nuclear Antigen Expression in Pulmonary Artery Smooth Muscle Cell Induced by PDGF

The effects ofRG50864 on DNA percentcontentand proliferation cellnuclear antigen (PCNA) in prim ary culture calfpulm onary artery sm ooth m uscle cell(PASMC) induced by PDGFw ere studied by using flow cytom etry. As com pared w ith controlgroup, fluorescence intensity and index ofPDGF- induced PASMC PCNA expression w ere significantly inhibited in RG50864 groups (P< 0.01). In RG50846 groups, DNApercent content ofG0/G1 phase w as increased, while thatofSand G2 M phase decreased (P< 0.01) . It was concluded thatRG50864 could significantly inhibitthe PDGF-induced PASMCPCNAexpression and block theconverting of cells from G 0/G 1 phase to Sphase ofDNA synthesis.