多发性骨髓瘤负性相关基因DAZAP2蛋白高效表达和纯化

目的在前期的研究中，DAZAP2基因在多发性骨髓瘤（Multiple myeloma，MM）患者中表达明显下调，可能为MM的负性基因．须进一步获得DAZAP2蛋白，制备抗体，从而在蛋白水平上研究DAZAP2的表达、结构和功能．方法将原核重组质粒pQE30－DAZAP2转化大肠杆菌，阳性菌株经1 mmol／LIPTG诱导表达目的蛋白，再经过纯化和透析结果IPTG诱导4hr时，得到大量重组目的蛋白，占可溶蛋白的20％左右，经过Ni—NTA层析柱纯化。获得分子量为17kD的DAZAP2蛋白浓度为0．97mg／m1．结论DAZAP2蛋白获得高效表达，并且建立快速简便的纯化方法，目的蛋白可以用于下一步的实验．

Highly Effective Expression and Purification of Multiple Myeloma Negative - related DAZAP2 Protein

Objective In former study,gene DAZAP2 is down-regulated expression in multiple myeloma patients.To gain a number of DAZAP2 protein to prepare antibody for further study the expression,structure and function of DAZAP2 protein.Methods The recombined prokaryotic expression vector pQE30-DAZAP2 was transformed into E.coil JM109 and induced by IPTG for expression.Results After 4 hours induced,a large of recombined protein were prepared,account for over 20% dissolved protein.The purified protein(around 17 kD) was gained by Ni affinity chromatography,it's concentration was about 0.97mg/ml.Conclusions DAZAP2 protein was highly and effectively expressed and rapidly and simply purified for further study.