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| 肺吸虫病金标渗滤试剂盒现场应用价值及流行病学调查 | |
| 引用本文： | 朱名胜,钱宝珍,杉山広,王绍基,宋明华. 肺吸虫病金标渗滤试剂盒现场应用价值及流行病学调查[J]. 山西医科大学学报, 2008, 39(12): 1090-1091 |
| 作者姓名： | 朱名胜 钱宝珍 杉山広 王绍基 宋明华 |
| 作者单位： | 1. 郧阳医学院寄生虫学教研室,十堰,442000 2. 浙江省医学科学院生物工程研究所 3. 日本国立感染症研究所,东京,1628640,日本 |
| 基金项目： | 日本健康科学基金资助项目 ， 浙江省科技厅基金资助项目 |
| 摘 要： | 目的 评价肺吸虫病金标渗滤法抗体快速检测试剂盒(DIGFA-kit)现场应用效果及调查肺吸虫病流行情况. 方法 应用DIGFA-kit检测流行区人群血清抗体,并与常规ELISA相比较;同时调查流行区中间宿主、动物宿主感染情况. 结果 共采集人群血清样本1 062份,阳性54份,阳性率为5.08%,ELISA平行对照试验阳性符合率为100%.第一中间宿主螺的感染率为0.64%,第二中间宿主淡水蟹感染为2.95个囊蚴/只,动物宿主猫的自然感染率为44.68%. 结论 DIGFA-kit具有较好的灵敏度和特异性,适合于流行区临床检验和大规模流行病学调查.调查区域有第一、第二中间宿主和保虫宿主的存在,为斯氏肺吸虫病流行地区. |
| 关 键 词： | 肺吸虫病 斯氏狸殖吸虫 免疫学试验 流行病学 |
Detection of paragonimiasis by dot immunogold filtration asser(DIGFA-kit) and investigation of epidemiology |
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| ZHU Ming-sheng,QIAN Bao-zhen,SHAN Shan-guang,WANG Shao-ji,SONG Ming-hua. Detection of paragonimiasis by dot immunogold filtration asser(DIGFA-kit) and investigation of epidemiology[J]. Journal of Shanxi Medical University, 2008, 39(12): 1090-1091 | |
| Authors: | ZHU Ming-sheng QIAN Bao-zhen SHAN Shan-guang WANG Shao-ji SONG Ming-hua |
| Affiliation: | 1Dept of Parasitology;Yunyang Medical College;Shiyan 442000;China;2Zhejiang Province Academy of Medical Sciences;3 Japanese National Institute of Infectious Diseases;Tokyo 1628640;Japan |
| Abstract: | Objective To appraise the effect of DIGFA-kit on field application,and to investigate the popular situation of paragonimiasis. Methods The serum antibody of the crowd in the popular area was detected by DIGFA-kit and compared with the result by ELISA.The infection situation of intermediate host and reservoir host in the popular area was investigated simultaneously. Results Of 1 062 serum samples collected from the crowd,54 were positive with the positive rate of 5.08%.The masculine coincidence rate was 100%... |
| Keywords: | Paragonimiasis Pagumogonimus skrjabini immunological test epidemiology |
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