| 氟对体外培养软骨细胞超微结构的影响 |
| |
| 引用本文： | 李良忠,张宏印,刘开泰.氟对体外培养软骨细胞超微结构的影响[J].中国地方病防治杂志,2009(3):167-169. |
| |
| 作者姓名： | 李良忠 张宏印 刘开泰 |
| |
| 作者单位： | 北京大学医院口腔中心;中国疾病预防控制中心教育培训处; |
| |
| 基金项目： | 国家自然科学基金资助项目(30560131) |
| |
| 摘 要： | 目的观察氟化物对体外培养乳鼠软骨细胞超微结构的影响。方法采用细胞培养的方法，原代培养昆明乳鼠软骨细胞，传第3代后按染氟剂量不同分为0（对照）、5、10、20、40mg／L组，染氟培养10d后，制备乳鼠软骨细胞超薄切片，透射电镜下观察软骨细胞的超微结构改变。结果电镜下5、10mg／L组软骨细胞增殖，双核细胞数量增多，细胞器发达，可见囊池扩张；20、40mg／L组细胞表面微绒毛减少，部分细胞染色质固缩，并凝结成块状，聚集在核膜周边，核基质密度下降，核膜迂回曲折，胞浆内线粒体空泡化，有些细胞胞浆内可见大量的囊泡，泡内含有电子致密物质；在40mg／L组中可见到凋亡细胞。结论高氟可以引起乳鼠软骨细胞超微结构的损伤。
|
| 关 键 词： | 氟 软骨细胞 超微结构 |
Effects of fluoride on ultrastructure of chondrocyte of culture in vitro |
| |
| LI Liang-zhong,ZHANG Hong-yin,LIU Kai-tai.Effects of fluoride on ultrastructure of chondrocyte of culture in vitro[J].Chinese Journal of Control of Endemic Disenaces,2009(3):167-169. |
| |
| Authors: | LI Liang-zhong ZHANG Hong-yin LIU Kai-tai |
| |
| Institution: | LI Liang-zhong,ZHANG Hong-yin,LIU Kai-tai(Department of stomatology,Hospital of Peking University,Peking 100871) |
| |
| Abstract: | Objective To investigate the effects of fluoride of ultra-structure of chondrocyte of culture in vitro.Methods The third generation of primary cultured chondrocytes were exposed to the concentration of 0(control),5,10,20,40 mg/L fluoride for 10 days.Chondrocytes of newborn mouse were collected and ultra-thin sections were made and chondrocyte ultra-styucture was observed under eletronic microscope.Results After exposed to fluoride for 10 days,the proliferation was present in the chondrocyte of 5,10 mg/L gro... |
| |
| Keywords: | Fluoride Chondrocyte Ultrastructure |
| 本文献已被 CNKI 维普 等数据库收录！ |
