Construction of genetically attenuated bacteria Bordetella pertussis devoid of dermonecrotic toxin activity that produces modified nontoxic form of pertussis toxin |
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| Authors: | L. N. Sinyashina L. S. Sinyashina E. G. Semin I. P. Amelina G. I. Karataev |
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| Affiliation: | (1) Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia;(2) State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia; |
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| Abstract: | Recombinant (attenuated) bacteria Bordetella pertussis that contains a knock-out mutation in dnt gene and produces a nontoxic derivative of pertussis toxin have been constructed. Immunological properties of mutant bacteria B. pertussis of KS strain have studied. It is demonstrated that recombinant bacteria B. pertussis of KS strain devoid of activity of dermonecrotic toxin protect the structure of mutant dnt genes upon cultivation on selective nutrient media and long-term exposure in organisms of laboratory animals. The intranasal immunization of mice with live B. pertussis bacteria of the attenuated KS strain protects animals from infection with virulent strain of pertussis pathogen, which can be comparable with an OSO-3 industrial standard sample. |
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