SPOP,ZEB-1 and E-cadherin expression in clear cell renal cell carcinoma (cc-RCC): Clinicopathological and prognostic significance |
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Authors: | Ola A. Harb Mariem A. Elfeky Basant Sh El Shafaay Heba F. Taha Gamal Osman Ibtsam Shehta Harera Loay M. Gertallah Doaa Metwaly Abdelmonem Ahmed Embaby |
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Affiliation: | 1. Department of Pathology, Zagazig University, Faculty of Medicine, Zagazig, Egypt;2. Department of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Zagazig, Egypt;3. Department of Medical Oncology, Faculty of Medicine, Zagazig, Egypt;4. Department of General surgery, Faculty of Medicine, Zagazig University, Zagazig, Egypt;5. Department of Clinical Pathology, Faculty of Medicine, Zagazig University, Zagazig, Egypt;6. Department of Internal Medicine, Faculty of Medicine, Zagazig University, Zagazig, Egypt |
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Abstract: | BackgroundClear cell renal cell carcinoma (cc-RCC), is a serious cancer regarding; its fatality, liability for metastases and chemoresistance, so identification of recent therapeutic targets to improve the patients prognosis is needed. SPOP is a BTB/POZ domain containing speckle-type POZ protein, has been identified as an E3 ubiquitin ligase component. ZEB1 is an essential epithelial mesenchymal transition (EMT) activator; E-cadherin is a cell adhesion protein that had been detected in normal epithelial cells membrane.AimWas to assess the tissue protein markers SPOP, ZEB1 & E-cadherin expressions in benign areas of neoplastic kidney specimens and in cc-RCC patients, then correlating their expression levels with patients clinicopathological and prognostic data.MethodsWe evaluated SPOP, ZEB-1 & E-cadherin expression using immunohistochemistry in samples from 50 cc-RCC and 20 benign areas of neoplastic kidney specimens, then we followed our patients for 5 years and finally we have analyzed correlations between the levels of markers expressions with patients clinicopathological and prognostic criteria in cc-RCC.ResultsPositive expression of SPOP & ZEB1 in addition to negative E- cadherin expression was detected in cc-RCC more than benign areas of neoplastic kidney specimens (p?=?0.004 and p?0.001 respectively). In cc-RCC Positive expression of SPOP, ZEB1 and negative E- cadherin expression was associated with higher grade (p?=?0.006, 0.007 & <0.001 respectively), advanced AJCC stage (p?=?0.013, 0.023 & <0.001 respectively), presence of L.N metastases (p?=?0.002?=?0.010 and <0.001 respectively), distant metastases (p?=?0.001, 0.003 & 0.035 respectively), poor PFS and OS rates (p?0.001 and p?=?0.013 respectively).ConclusionPositive expression of SPOP& ZEB1 in addition to negative E- cadherin are associated with poor prognosis in cc-RCC patients. |
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Keywords: | cc-RCC SPOP ZEB1 E-cadherin Immunohistochemistry Prognosis |
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