| 槲皮素对胃癌MGC-803细胞生长及凋亡作用的研究 |
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| 引用本文： | 王海燕,郭良淼,陈勇,赵雪花,成彩莲,吴勉云,何丽娅.槲皮素对胃癌MGC-803细胞生长及凋亡作用的研究[J].中国药房,2006,17(19):1455-1456. |
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| 作者姓名： | 王海燕 郭良淼 陈勇 赵雪花 成彩莲 吴勉云 何丽娅 |
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| 作者单位： | 武汉科技大学医学院,武汉市,430080 |
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| 摘 要： | 目的研究槲皮素对人胃癌MGC-803细胞增殖的影响及诱导凋亡作用。方法用四甲基偶氮唑盐(MTT)比色法检测不同浓度槲皮素对MGC-803细胞的增殖活性的效应;原位末端核苷标记(TUNEL)法检测槲皮素诱导细胞凋亡的凋亡指数(AI)。结果槲皮素浓度为40~100μmol/L时对MGC-803细胞增殖有显著的抑制效应,且呈剂量依赖性;细胞加入槲皮素诱导48h后AI明显高于未加入组(P<0.05)。结论槲皮素在一定浓度范围内能显著抑制MGC-803细胞的增殖并能诱导其凋亡,其作用呈浓度依赖性。
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| 关 键 词： | 槲皮素 MGC-803细胞 增殖 抑制作用 凋亡 |
| 文章编号： | 1001-0408(2006)19-1455-02 |
| 收稿时间： | 12 22 2005 12:00AM |
| 修稿时间： | 03 7 2006 12:00AM |
Study on Growth Effect and Apoptosis Action of Quercetin on Human Gastric Carcinoma Cells MGC- 803 |
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| WANG Haiyan,GUO Liangmiao,CHEN Yong,ZHAO Xuehua,CHENG Cailian,WU Mianyun,HE Liya.Study on Growth Effect and Apoptosis Action of Quercetin on Human Gastric Carcinoma Cells MGC- 803[J].China Pharmacy,2006,17(19):1455-1456. |
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| Authors: | WANG Haiyan GUO Liangmiao CHEN Yong ZHAO Xuehua CHENG Cailian WU Mianyun HE Liya |
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| Institution: | College of Medical Science,Wuhan University of Science and Technology,Wuhan 430080,China |
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| Abstract: | OBJECTIVE:To study the effect of quercetin on the proliferation and apoptosis of human gastric carcinoma cell line MGC-803.METHODS:MTT assay was adopted to measure the effect of quercetin at different concentrations on the proliferation of MGC-803 cells.Cell apoptosis index(AI)induced by quercetin was measured by TUNEL assay.RESULTS: Quercetin at a certain concentration from 40μmol/L to 100μmol/L could successfully inhibit the proliferation of MGC-803 cells in a dose-dependent manner.TUNEL assay indicated that apoptosis cells induced by quercetin in treatment group were much more than that in control group after 48 hours(P<0.05).CONCLUSIONS:Quercetin of certain concentration can evidently inhibit the proliferation of MGC-803 cells and induce their apoptosis with concentration-dependent relation. |
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| Keywords: | Quercetin MGC 803 cells Proliferation Inhibitory effect Apoptosis |
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