| PCR检测恶性疟原虫感染蚊方法的建立 |
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| 引用本文： | 郑彬,刘燕,武松,王学忠. PCR检测恶性疟原虫感染蚊方法的建立[J]. 国际医学寄生虫病杂志, 2007, 34(3): 119-121 |
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| 作者姓名： | 郑彬 刘燕 武松 王学忠 |
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| 作者单位： | 1. 200025,上海,中国疾病预防控制中心寄生虫病预防控制所,世界卫生组织疟疾、血吸虫病和丝虫病合作中心,卫生部寄生虫病原与媒介生物学重点实验室
2. 思茅,665000,云南省寄生虫病预防控制所 |
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| 摘 要： | 目的 建立一种简便的可应用于现场蚊感染恶性疟原虫的PCR检测方法。方法 采用针对恶性疟原虫小亚单位核糖体DNA（SSUrDNA）的特异引物，利用PCR技术，从实验室感染及现场采集的感染恶性疟原虫的蚊基因组DNA中，扩增恶性疟原虫SSUrDNA片段，进行恶性疟原虫的检测。结果 扩增产物经琼脂糖凝胶电泳分析，可检测出特异的约188bp大小的DNA片段，其基因序列与预计序列完全一致。估测每份蚊DNA样本中含有10个以上子孢子即可获得此片段，而对间日疟原虫及未感染蚊DNA不能扩增出此片段。结论 该PCR检测方法可应用于现场恶性疟原虫感染蚊的检测。
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| 关 键 词： | 恶性疟原虫 蚊 PCR |
| 修稿时间： | 2007-03-09 |
Development of a PCR-based method for detection of Plasmodium falciparum infected mosquitoes |
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| ZHENG Bin,LIU Yan,WU Song,WANG Xue-zhong. Development of a PCR-based method for detection of Plasmodium falciparum infected mosquitoes[J]. International JOurnal of Medical Parasitic Diseases, 2007, 34(3): 119-121 |
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| Authors: | ZHENG Bin LIU Yan WU Song WANG Xue-zhong |
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| Affiliation: | 1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Key Laboratory of Parasite and Vector Biology, MOH , Shanghai 200025, China ;2. institute of Parasitic Diseases of Yunnan Province, Simao 665000, China |
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| Abstract: | Objective To develop a PCR-based method for detection of Plasmodiumfalciparum infected mosquitoes. Methods One pair of primers specific to small subunit ribosomal DNA of P. faciparum were used to amplify the specific SSUrDNA 188 bp fragment of P. faciparum for detecting P. fnciparum infected mosquitoes with PCR-based method. Results As few as 10 sporozoites in one mosquito no matter collected in the field or infected in the lab could be detected with the PCR-based method. In contrast, no such specific 188 bp DNA band was detected in P. vivax and uninfected mosquito. Conclusion The PCR-based method can be used to detect P. faciparum infected mosquitoes collected in the field. |
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| Keywords: | Plasmodium falciparum Mosquito PCR |
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