| 狂犬病毒aG株糖蛋白膜外区基因克隆和序列分析 |
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| 引用本文： | 明文玉,黎孟枫.狂犬病毒aG株糖蛋白膜外区基因克隆和序列分析[J].广东药学院学报,1995,11(3):146-151. |
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| 作者姓名： | 明文玉 黎孟枫 |
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| 作者单位： | 广东省生物技术研究所，病毒基因工程国家重点实验室，卫生部武汉生物制品研究所 |
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| 摘 要： | 本文应用聚合酶链反应技术获得了狂犬病毒ａＧ株糖蛋白膜外区基因克隆并进行了序列分析。测出其核苷酸序列与其他株相应区序列的同源性在９０．１２％到９３．０３％之间，推导氮基酸序列的同源性在９１．４１％到９３％、２３％之间，经分析发现成熟肽推导氨基酸第１８２位的替代，导致了乙酰胆碱受体结合序列的构象改变，可能是造成ＲＶａＧ毒力减弱的分子基础。
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| 关 键 词： | 狂犬病毒 糖蛋白基因 聚合酶链反应 基因克隆 |
Molecular Cloning and seguencing of the Glycoprotein Ectodomain Gene of a G Strain of Rabies Virus |
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| Ming Wenyu,Li Mengfeng,Zhu Jiahong,Hou Yunde.Molecular Cloning and seguencing of the Glycoprotein Ectodomain Gene of a G Strain of Rabies Virus[J].Academic Journal of Guangdong College of Pharmacy,1995,11(3):146-151. |
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| Authors: | Ming Wenyu Li Mengfeng Zhu Jiahong Hou Yunde |
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| Abstract: | we have cloned and sequenced the glycoprotein ectodomain gene of aG strain of rabies virus(RVaG)by using polymerasc chain reaction(PCR)method. Compering its nucleotide scquences with the corresponding sequences of other strains revealed that the gornologies of the nucleotide se-quences were between 90.12%(CVS/aG)and 93.03% (SADB19./aG)and the homologies of the de-duced amino acid sequences between 991.4%(CVS/aG)and 93.23%(PV/aG).By comparing and analyzing,we bdteved that the replacements on 182 of the deduced amino acid in the matured peptide of the glycoprotein had resulted in the configuration change of the sequence linking to acetyl-choline receptor (AChR),wbich could have reduced the virulence of RVaG. |
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| Keywords: | rabies virus glycoprotein gene polymerasechain reaction molecular cloning se-quencing(PCR) |
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