| 人脑肌酸激酶单克隆抗体的制备及鉴定 |
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| 引用本文： | 曹岩,刘玉鹏,陈培勋,修波,曹惠婷,张祖传.人脑肌酸激酶单克隆抗体的制备及鉴定[J].青岛大学医学院学报,1995(1). |
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| 作者姓名： | 曹岩 刘玉鹏 陈培勋 修波 曹惠婷 张祖传 |
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| 作者单位： | 中国科学院上海生物化学研究所 |
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| 摘 要： | 用事故死亡者脑组织中提纯的人脑肌酸激酶（ＣＫ－ＢＢ）对ＢＡＬＢ／Ｃ小鼠进行免疫，应用杂交瘤技术将免疫小鼠脾细胞分别与２种骨髓瘤细胞（ＮＳ－１，６５３）融合，筛选得到８株能稳定分泌ＩｇＭ抗体的杂交瘤细胞。对分泌抗体经酶联免疫电泳转移试验（Ｗｅｓｔ－Ｂｌｏｔ）进行了鉴定，其中４种抗体能特异地与ＣＫ－ＢＢ的ＳＤＳ－ＰＡＧＥ电泳带结合。提示这些单克隆抗体有可能应用于肌酸激酶的结构功能研究和临床检验中。
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| 关 键 词： | 肌酸激酶类，抗体，单克隆，杂交瘤，小鼠，近交BALB／C |
ESTABLISHMENT AND IDENTIFICATION OF A HYBRIDOMA CELL LINE SECRETING McAb AGAINST CK-BB |
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| Cao Yan,Liu Yupeng,Chen Peixun,et al.ESTABLISHMENT AND IDENTIFICATION OF A HYBRIDOMA CELL LINE SECRETING McAb AGAINST CK-BB[J].Acta Academiae Medicinae Qingdao Universitatis,1995(1). |
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| Authors: | Cao Yan Liu Yupeng Chen Peixun |
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| Institution: | Department of Biochemistry |
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| Abstract: | Spleen cells from BALB/C mice immunized with purified human brain creatine kinase(CK-BB) were fused separately with 653 and NS-1 myeloma cells.Eight hybridoma cell lines secreting McAb against human CK-BB were obtained after clonings.Their specifications were identified by enzyme-linked immunoelectrotransfer blot techniques(West-Blot).The McAbs were identified as mouse IgM.The McAbs can be applied to the clinical laboratory test of creatine kinase and research about CK-BB. |
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| Keywords: | creatine kinase antibodies monoclonal hybridomase mice inbred BALB C |
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