补气活血方对慢性支气管炎肺气虚证大鼠肺组织bcl-2、bax表达的影响

目的：观察补气活血方对慢性支气管炎肺气虚证大鼠肺组织 bcl-2及 bax表达的影响。方法 SD大鼠50只，随机选取10只作为空白对照组，其余40只采用改良烟熏法复制慢性支气管炎肺气虚证大鼠模型，造模成功后随机分为模型组、补气活血方高剂量组、补气活血方低剂量组、补肺汤组，每组10只。采用免疫组化法检测细胞凋亡相关基因蛋白 bcl-2、bax的表达情况。结果与空白对照组比较，模型组大鼠 bcl-2表达明显增加，bax表达降低（ P 〈0.01）；与模型组比较，补气活血方高剂量组、补气活血方低剂量组、补肺汤组 bcl-2蛋白表达降低，bax 蛋白表达增加（ P〈0.01）；补气活血方高剂量组 bcl-2的表达较低剂量组和补肺汤组明显减少，bax的表达较低剂量组和补肺汤组明显增多（P〈0.01）；补气活血方低剂量组 bcl-2表达低于补肺汤组，bax表达高于补肺汤组（P 〈0.05）。结论补气活血方能够通过抑制细胞凋亡相关基因蛋白 bcl-2的表达，上调蛋白 bax的表达，促进炎性细胞凋亡，可作为预防和治疗慢性支气管炎的有效方法。

Effect of the Buqi Huoxue Decoction on Expressions of bcl-2 and bax in Chronic Bronchitis Rats with Deficiency of Lung-qi

Objective To observe the effect of the Buqi huoxue decoction on expressions of bcl-2 and bax in chronic bronchitis（ CB）rats with deficiency of lung-qi. Methods Ten rats were randomly selected from 50 SD rats as control group,and models of the deficiency of lung-qi of chronic bronchitis were established using the improved smoking-fumigated method in the other 40 rats. The successful model rats were randomly divided into model group,high-dose group of Buqi huoxue decoction,low-dose group of Buqi huoxue decoction and tonifying lung recipe group（ n =10 for each group）. Expressions of apoptosis-related gene bcl-2 and bax were detected using immunohistochemistry method. Re-sults Compared with those in control group,the bcl-2 expression in the model group was significantly increased,while the bax expression was significantly decreased（P〈0. 01）;compared with those in model group,the bcl-2 expressions were significantly reduced,while the bax expressions were significantly increased in high-dose,low-dose and tonifying lung recipe groups（P〈0. 01）;the bcl-2 expression in high dose group was significantly decreased,while the bax ex-pression in high dose group was significantly increased than those in low dose and tonifying lung recipe groups（ P 〈0. 01）;the bcl-2 expression was significantly lower,while the bax expression was significantly higher in low-dose group than that in tonifying lung recipe group（P〈0. 05）. Conclusion The Buqi huoxue decoction can inhibit the expression of apoptosis-related gene bcl-2,increase the expression of bax in inflammatory cells and promote inflammatory cell apop-tosis,which can be used as an effective method of prevention and treatment of chronic bronchitis.