| 丹参对体外培养大鼠成纤维样滑膜细胞bcl-2和bax表达的影响 |
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| 引用本文： | 任威铭,张葆花,吴承艳,佟书娟.丹参对体外培养大鼠成纤维样滑膜细胞bcl-2和bax表达的影响[J].华北国防医药,2014,0(9):13-16. |
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| 作者姓名： | 任威铭 张葆花 吴承艳 佟书娟 |
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| 作者单位： | 任威铭 (南京中医药大学基础医学院, 南京,210023)； 张葆花 (石家庄市中医院风湿科, 石家庄,050051)； 吴承艳 (南京中医药大学 文献研究所, 南京,210023)； 佟书娟 (南京中医药大学 微生物教研室, 南京,210023)； |
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| 基金项目： | 江苏省自然科学基金资助项目(BK20131418)；江苏省高等学校大学生实践创新训练计划项目(项目编号：2012JSSPITP1130) |
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| 摘 要： | 目的：探讨丹参对体外培养大鼠成纤维样滑膜细胞增殖及 bax、bcl-2表达的影响。方法取浓度为1.5＆#215;10-5个/ml的 RSC-364成纤维样滑膜细胞悬液100μl加入细胞培养板，培养24 h，加入含不同浓度丹参的培养液，采用 MTT法分析丹参对成纤维样滑膜细胞增殖抑制作用及其时效关系；免疫组化法检测 bcl-2、bax表达情况。结果丹参可明显抑制大鼠成纤维样滑膜细胞增殖，并显示出一定的量效和时效关系。丹参可显著上调大鼠成纤维样滑膜细胞 bax表达，下调 bcl-2表达，与空白对照组比较差异有统计学意义（ P 〈0.05）。结论丹参对体外培养大鼠成纤维样滑膜细胞的增殖具有抑制效应，其作用机制可能与调控 bax、bcl-2表达有关。
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| 关 键 词： | 丹参 成纤维细胞 细胞增殖 大鼠 |
Effect of Salvia Miltiorrhiza on Fibroblastic Synovial Cells of bax/bcl-2 Expressions of Culture in Rats in Vitro |
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| Institution: | REN Wei-ming, ZHANG Bao-hua, WU Cheng-yan, TONG Shu-juan (a. Department of Clinical Profession of Chinese and Western Medicines of Basic Medical College, b. Literature Research Institute, c. Department of Microbiology, Nanjing, 1. Nanjing University of Chinese Medicine, Nanjing 210023, China; 2. Department of Rheumatology, Traditional Chinese Medical Hospital of Shijiazhuang City, Shijiazhuang 050051, China) |
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| Abstract: | Objective To explore the effect of Salvia Miltiorrhiza on fibroblastic synovial cells of bax/bcl-2 ex-pressions and proliferation culture in rats in vitro. Methods A concentration of 1. 5 ＆#215; 10 -5/ ml RSC-364 fibroblastic synovial cells of 100 ul cell suspension was added with the cell culture plate,and was cultivated for 24 h,and then differ-ent concentrations of Salvia Miltiorrhiza culture medium were added. Inhibition effect of Salvia Miltiorrhiza on fibroblastic synovial cell proliferation and its time-effect relationship were analyzed using MTT method;bcl-2 and bax expressions were detected using immunohistochemical method. Results Salvia Miltiorrhiza was able to inhibit the cell proliferation of fibroblastic synovial cells in rats,and it showed a certain degree of concentration-response and time-effect relationships. Salvia Miltiorrhiza could significantly increase the bax expression of fibroblastic synovial cells,and reduce bcl-2 expres-sion of synovial cells,and the differences,compared with those in control group,were statistically significant（ P 〈0. 05）. Conclusion Salvia Miltiorrhiza can inhibit the proliferation of fibroblastic synovial cell culture in rats in vitro , and its mechanism of action may relate to regulation of bax and bcl-2 expressions. |
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| Keywords: | Salvia miltiorrhiza Fibroblasts Cell proliferation Rats |
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