| 高效液相色谱法测定金毛耳草中芦丁的含量 |
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| 引用本文： | 吴孔松,吴安明,支永英.高效液相色谱法测定金毛耳草中芦丁的含量[J].海峡药学,2014,0(8):44-46. |
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| 作者姓名： | 吴孔松 吴安明 支永英 |
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| 作者单位： | 吴孔松(江西天施康中药股份有限公司鹰潭335000)；吴安明(江西天施康中药股份有限公司鹰潭335000)；支永英(江西天施康中药股份有限公司鹰潭335000)； |
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| 摘 要： | 目的采用HPLC法测定金毛耳草中芦丁的含量。方法采用Dikma C18色谱柱（250mm×4.6mm,5μm）,流动相为乙腈-0.3%冰醋酸（18∶82）,流速1.0mL·min-1,检测波长350nm,柱温：25℃。结果芦丁的质量浓度在5.3μg·mL-1~85.0μg·mL-1范围内与峰面积呈良好线性关系,平均回收率为97.88%,RSD为0.75%。结论本方法简便、准确,重复性好,可作为金毛耳草药材的质量控制方法。
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| 关 键 词： | 高效液相色谱 金毛耳草 芦丁 含量测定 |
Determination of Rutin in Hedyotis Chrysoticha by HPLC |
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| WU Kong-song,WU An-ming,ZHI Yong-ying.Determination of Rutin in Hedyotis Chrysoticha by HPLC[J].Strait Pharmaceutical Journal,2014,0(8):44-46. |
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| Authors: | WU Kong-song WU An-ming ZHI Yong-ying |
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| Institution: | (Jiangxi Tian Shi Kang Chinese Herbal Drugs Limited Com- pany, Yingtan 33.5000, China) |
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| Abstract: | OBJECTIVE To establish a method for the determination of rutin in Hedyotis chrysoticha by HPLC. METHODS The separation was performed on a Dikma C18column（ 250 mm × 4. 6mm,5μm） with a mobile phase of acetonitrile-0. 3% acetic acid（ 18 ∶ 82）. The flow rate was 1. 0mL·min- 1. The detection wavelength was350 nm and the column temperature was 25℃. RESULTS The calibration cure of rutin was performed over the range of 5. 3μg·mL- 1~ 85. 0μg·mL- 1. The average recovery was 97. 88% with RSD of 0. 75%. CONCLUSION The method is simple and accurate with good reproducibility,and can be used for the quality control of Hedyotis chrysoticha. |
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| Keywords: | HPLC Hedyotis chrysoticha Rutin Determination |
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