| HPLC法测定不同种类茶叶咖啡因含量 |
| |
| 引用本文： | 葛静,刘洪旭,邓思珊. HPLC法测定不同种类茶叶咖啡因含量[J]. 海峡药学, 2014, 0(8): 57-59 |
| |
| 作者姓名： | 葛静 刘洪旭 邓思珊 |
| |
| 作者单位： | 葛静 (福建省福州市第八医院福州350013); 刘洪旭 (福建省医学科学研究院福州350001); 邓思珊 (福建省医学科学研究院福州350001); |
| |
| 摘 要： | 目的采用高效液相色谱法测定8种茶叶中咖啡因的含量。方法采用Ultimate XB-C18（4.6×250mm,5μm）色谱柱,流动相：乙腈-0.2%磷酸水溶液,梯度洗脱,流速1.0mL·min-1,检测波长为280nm,进样量20μL。结果在所选条件下,样品中待测组分与其它组分分离良好（R〉1.5）,咖啡因对照品在10-100μg·mL-1质量浓度范围内线性关系良好,r=0.9998;回收率为98.8%-102.5%,RSD=1.6%（n=6）。结论该方法操作简便,结果可靠,适合用于茶叶中咖啡因定量分析。
|
| 关 键 词： | 高效液相色谱法 武夷茶 咖啡因 |
Determination of the Content of Caffeine in Different Kinds of Tea by HPLC |
| |
| Affiliation: | GE Jing ,LIU Hong-xu,DENG Si-shan(1. The eigth hospital of Fujian Fuzhou,Fuzhou 3500]3,China; 2. Fujian academy of Medical Sciences, Fuzhou 350001 ,China) |
| |
| Abstract: | OBJECTIVE To analyze the content of CAF in the eight kinds of tea by High-performance Liquid Chromatography. METHODS Using a Ultimate XB-C18（ 4.6 ×250mm,5μm） as the chromatographic column,with the moving phase of acetonitrile： 0. 2% phosphoric acid solution gradient elution,the velocity of flow was 1. 0mL·min- 1,the UV detection wavelength was 280 nm,the sample dose was 20μL. RESULTS Under the selected condition,the aim ingredient in the samples got an ideal resolution with others（ the resolution was above 1. 5）. Caffeine showed good linear relationship in the concentration range of 10 - 100μg·mL- 1,r =0.9998. The recovery rate was98. 8% to 102. 5%,RSD was 1. 6%（ n = 6）. CONCLUSION The method is simple and result is reliable,which can be used for quantitative analysis of caffeine in tea. |
| |
| Keywords: | HPLC Tea Caffeine |
| 本文献已被 维普 等数据库收录！ |
|
