脑卒中患者基质金属蛋白酶基因多态性研究

目的探讨基质金属蛋白酶(MMP)-2和MMP-9基因多态性位点与脑卒中的关系。方法选择脑卒中患者348例为脑卒中组和健康体检者235例为对照组,采用限制性片段长度多态性分析技术检测MMP-2基因C1306T、C735T和MMP-9基因C1562T多态的分布特点并进行分析。根据诊断又将脑卒中患者分为脑出血组(116例)、脑梗死组(115例)、脑栓塞组(31例)、腔隙性脑梗死组(86例)。结果与对照组比较,脑卒中组收缩压、舒张压和TG水平明显升高,吸烟、颈动脉粥样硬化比例明显升高,差异有统计学意义(P<0.05)。脑出血组和脑梗死组MMP-9 C1562T的CT+TT基因型频率和T等位基因频率明显高于对照组(P<0.05)。脑梗死组MMP-2C735T的CC基因型频率和C等位基因频率明显高于对照组(P<0.05)。MMP-2、MMP-9各基因型均不是脑卒中预后影响因素。结论 MMP-2 C735T的C等位基因、MMP-9 C1562T的T等位基因是脑梗死的遗传易感基因之一,同时后者也是脑出血的遗传易感基因之一。MMP-2、MMP-9基因多态性与脑卒中预后无关。

Study on polymorphisms of matrix metalloproteinase-2 and -9 genes of stroke patients

Objective To investigate the relation of gene polymorphisms of matrix metalloprotein-ase-2 and matrix metalloproteinase-9 to stroke.Methods 348 patients with stroke(stroke group) and 235 healthy controls(control group) were recruited.PCR-RFLP was used to detect C1306T, C735T polymorphism of MMP-2 gene and C1562T polymorphism of MMP-9 gene.The stroke patients were divided into cerebral hemorrhage group,cerebral infarction group,cerebral embolism group and lacunar infarction group according to diagnosis.Results Levels of systolic pressure,diastolic pressure,triglyceride and proportion of history of smoking,carotid atherosclerosis were obviously higher in stroke group than in controls(P<0.05).The CT and TT genotype frequencies and T allele of C1562T of MMP-9 gene were higher in cerebral hemorrhage and cerebral infarction groups than in controls(P<0.05).The CC genotype frequency and C allele of C735T of MMP-2 gene were higher in cerebral infarction group than in controls(P<0.05).Different genotypes of MMP-2 and MMP-9 were not influential factor of prognosis of stroke.Conclusions These results suggest that the C allele of C735T of MMP-2 gene and T allele of C1562T of MMP-9 gene are one of genetic susceptible genes to cerebral infarction,the later is one of genetic susceptible genes to cerebral hemorrhagic.Polymorphisms of matrix metalloproteinase-2 and-9 genes have no relation with prognosis of stroke.